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Sm-D2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4511246

MBS4511246 is an antibody targeting Sm-D2. Also known as Rabbit anti-Sm-D2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$270 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Sm-D2, Polyclonal Antibody
Full Product Name
Rabbit anti-Sm-D2 Antibody
Product Gene Name
Product Synonym Names
SNRPD1; Small nuclear ribonucleoprotein Sm D2; Sm-D2; snRNP core protein D2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
118
OMIM
601061
3D Structure
ModBase 3D Structure for P62316
Immunogen
Recombinant full length protein of human Sm-D2

Product Categories/Family for anti-Sm-D2 antibody

ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Sm-D2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-Sm-D2 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF:IC: 1:50-1:200

Western Blot (WB) of anti-Sm-D2 antibody

Western blot analysis of Sm-D2 expression in HepG2 (A), Jurkat (B), mouse testis (C), mouse heart (D), rat brain (E) whole cell lysates.
Rabbit Anti-Sm-D2 Polyclonal Antibody – Cat# MBS4511246

Immunohistochemistry (IHC) of anti-Sm-D2 antibody

Immunohistochemical analysis of Sm-D2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-Sm-D2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4511246

Immunofluorescence (IF) of anti-Sm-D2 antibody

Immunofluorescent analysis of Sm-D2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-Sm-D2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4511246

Other Notes

Small volumes of anti-Sm-D2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Sm-D2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Small nuclear ribonucleoprotein Sm D2
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
NCBI Official Symbol
SNRPD2
NCBI Official Synonym Symbols
SMD2; Sm-D2; SNRPD1
UniProt Protein Name
Small nuclear ribonucleoprotein Sm D2
UniProt Synonym Protein Names
snRNP core protein D2
UniProt Gene Name
SNRPD2
UniProt Synonym Gene Names
SNRPD1; Sm-D2
UniProt Entry Name
SMD2_HUMAN
NCBI Protein Information
small nuclear ribonucleoprotein Sm D2; snRNP core protein D2

NCBI Summary for Sm-D2

The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
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UniProt Comments for Sm-D2

snRNP D2: Required for pre-mRNA splicing. Required for snRNP biogenesis. Belongs to the snRNP core protein family.

Protein type: RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; snRNP U1; cytosol; U12-dependent spliceosome

Molecular Function: protein binding

Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; gene expression
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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