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SPATA7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758911

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPATA7, Polyclonal Antibody
Full Product Name
Anti-SPATA7 Antibody Picoband
Product Gene Name
anti-SPATA7 antibody
Product Synonym Names
SET domain bifurcated histone lysine methyltransferase 2; Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2; C13orf4; CLLD8; KMT1F
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
HighlyExpressed in reproductive organs, such as testis, ovary and prostate.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
67,719 Da
OMIM
609868
3D Structure
ModBase 3D Structure for Q9P0W8
Immunogen
E coli-derived human SPATA7 recombinant protein (Position: R5-V599).
Subcellular Localization
Nucleus. Chromosome.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

Product Categories/Family for anti-SPATA7 antibody

Primary Antibodies; Immune System Diseases; Immunology; Protein Trafficking; Signal Transduction; Signaling Pathway
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SPATA7 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-SPATA7 antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-SPATA7 antibody

Figure 1. Western blot analysis of SPATA7 using anti-SPATA7 antibody (MBS1758911).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA7 antigen affinity purified polyclonal antibody (#MBS1758911) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SPATA7 at approximately 68 kDa. The expected band size for SPATA7 is at 68 kDa.
Rabbit Anti-SPATA7 Polyclonal Antibody – Cat# MBS1758911

Flow Cytometry (FC/FACS) of anti-SPATA7 antibody

Figure 2. Flow Cytometry analysis of HepG2 cells using anti-SPATA7 antibody (MBS1758911).
Overlay histogram showing HepG2 cells stained with MBS1758911 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA7 Antibody (MBS1758911, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-SPATA7 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758911

Product References and Citations for anti-SPATA7 antibody

1. Avila-Fernandez, A., Corton, M., Lopez-Molina, M. I., Martin-Garrido, E., Cantalapiedra, D., Fernandez-Sanchez, R., Blanco-Kelly, F., Riveiro-Alvarez, R., Tatu, S. D., Trujillo-Tiebas, M. J., Garcia-Sandoval, B., Ayuso, C., Cremers, F. P. M. Late onset retinitis pigmentosa. Ophthalmology 118: 2523-2524, 2011. 2. Eblimit, A., Agrawal, S. A., Thomas, K., Anastassov, I. A., Abulikemu, T., Moayedi, Y., Mardon, G., Chen, R. Conditional loss of Spata7 in photoreceptors causes progressive retinal degeneration in mice. Exp. Eye Res. 166: 120-130, 2018. Note: Erratum: Exp Eye Res. 171: 119, 2018. 3. Eblimit, A., Nguyen, T.-M. T., Chen, Y., Esteve-Rudd, J., Zhong, H., Letteboer, S., Van Reeuwijk, J., Simons, D. L., Ding, Q., Wu, K. M., Li, Y., Van Beersum, S., and 10 others. Spata7 is a retinal ciliopathy gene critical for correct RPGRIP1 localization and protein trafficking in the retina. Hum. Molec. Genet. 24: 1584-601, 2015.

Other Notes

Small volumes of anti-SPATA7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPATA7 purchase

MBS1758911 is an antibody targeting SPATA7. Also known as Anti-SPATA7 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPATA7. It may not necessarily be applicable to this product.

NCBI GI #
94536850
NCBI GeneID
55812
NCBI Accession #
NP_001035518.1
UniProt Primary Accession #
Q9P0W8
UniProt Secondary Accession #
Q5BKY5; Q8WX30; Q96HF3; Q9H0X0; Q9P0W7
UniProt Related Accession #
Q9P0W8
GenBank Nucleotide #
NM_001040428.3
NCBI Official Full Name
spermatogenesis-associated protein 7 isoform 2
NCBI Official Synonym Full Names
spermatogenesis associated 7
NCBI Official Symbol
SPATA7
NCBI Official Synonym Symbols
HSD3; LCA3; HSD-3.1; HEL-S-296
UniProt Protein Name
Spermatogenesis-associated protein 7
UniProt Synonym Protein Names
HSD-3.1; Spermatogenesis-associated protein HSD3
Protein Family
Spermatogenesis-associated protein
UniProt Gene Name
SPATA7
UniProt Synonym Gene Names
HSD3
UniProt Entry Name
SPAT7_HUMAN
NCBI Protein Information
spermatogenesis-associated protein 7; epididymis secretory protein Li 296; spermatogenesis-associated protein HSD3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SPATA7 antibody

 Disease Name  Pubmed Publications
 Leber Congenital Amaurosis Antibodies  >6 publications with SPATA7 and Leber Congenital Amaurosis
 Retinitis Pigmentosa Antibodies  >4 publications with SPATA7 and Retinitis Pigmentosa
 Neoplasms Antibodies  >3 publications with SPATA7 and Neoplasms
 Prostatic Diseases Antibodies  >2 publications with SPATA7 and Prostatic Diseases
 Genital Diseases, Male Antibodies  >2 publications with SPATA7 and Genital Diseases, Male
 Leber congenital amaurosis type 3 Antibodies  >1 publications with SPATA7 and Leber congenital amaurosis type 3

Organs/Tissues associated with anti-SPATA7 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >4 publications with SPATA7 and Eye
 Blood Antibodies  >2 publications with SPATA7 and Blood
 Prostate Antibodies  >2 publications with SPATA7 and Prostate
 Testis Antibodies  >2 publications with SPATA7 and Testis
 Adrenal Gland Antibodies  >1 publications with SPATA7 and Adrenal Gland
 Connective Tissue Antibodies  >1 publications with SPATA7 and Connective Tissue
 Brain Antibodies  >1 publications with SPATA7 and Brain
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