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SUCLA2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758528

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SUCLA2, Polyclonal Antibody
Full Product Name
Anti-SUCLA2 Antibody Picoband
Product Gene Name
anti-SUCLA2 antibody
Product Synonym Names
mediator complex subunit 14 ; Mediator of RNA polymerase II transcription subunit 14; Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; CRSP complex subunit 2; Mediator complex subunit 14; RGR1 homolog; hRGR1; Thyroid hormone receptor-associated protein complex 170 kDa component; Trap170; Transcriptional coactivator CRSP150; Vitamin D3 receptor-interacting protein complex 150 kDa component; DRIP150; MED14; ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Ubiquitous.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
48,040 Da
OMIM
603921
3D Structure
ModBase 3D Structure for Q9P2R7
Immunogen
E coli-derived human SUCLA2 recombinant protein (Position: Q50-H454).
Subcellular Localization
Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Product Categories/Family for anti-SUCLA2 antibody

Primary Antibodies; Epigenetics and Nuclear Signaling; Mediator Complex; Transcription
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SUCLA2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-SUCLA2 antibody
WB: 0.1-0.25ug/ml, Human, Mouse, Rat
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-SUCLA2 antibody

Figure 1. Western blot analysis of SUCLA2 using anti-SUCLA2 antibody (MBS1758528).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLA2 antigen affinity purified polyclonal antibody (#MBS1758528) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SUCLA2 at approximately 48 kDa. The expected band size for SUCLA2 is at 48 kDa.
Rabbit Anti-SUCLA2 Polyclonal Antibody – Cat# MBS1758528

Immunofluorescence (IF) of anti-SUCLA2 antibody

Figure 2. IF analysis of SUCLA2 using anti-SUCLA2 antibody (MBS1758528).
SUCLA2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-SUCLA2 Antibody (MBS1758528) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-SUCLA2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758528

Flow Cytometry (FC/FACS) of anti-SUCLA2 antibody

Figure 3. Flow Cytometry analysis of THP-1 cells using anti-SUCLA2 antibody (MBS1758528).
Overlay histogram showing THP-1 cells stained with MBS1758528 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLA2 Antibody (MBS1758528, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-SUCLA2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758528

Product References and Citations for anti-SUCLA2 antibody

1. Carrozzo, R., Dionisi-Vici, C., Steuerwald, U., Lucioli, S., Deodato, F., Di Giandomenico, S., Bertini, E., Franke, B., Kluijtmans, L. A. J., Meschini, M. C., Rizzo, C., Piemonte, F., Rodenburg, R., Santer, R., Santorelli, F. M., van Rooij, A., Vermunt-de Koning, D., Morava, E., Wevers, R. A. SUCLA2 mutations are associated with mild methylmalonic aciduria, Leigh-like encephalomyopathy, dystonia, and deafness. Brain 130: 862-874, 2007.2. Elpeleg, O., Miller, C., Hershkovitz, E., Bitner-Glindzicz, M., Bondi-Rubinstein, G., Rahman, S., Pagnamenta, A., Eshhar, S., Saada, A. Deficiency of the ADP-forming succinyl-CoA synthase activity is associated with encephalomyopathy and mitochondrial DNA depletion. Am. J. Hum. Genet. 76: 1081-1086, 2005. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/29/2015.

Other Notes

Small volumes of anti-SUCLA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SUCLA2 purchase

MBS1758528 is an antibody targeting SUCLA2. Also known as Anti-SUCLA2 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SUCLA2. It may not necessarily be applicable to this product.

NCBI GI #
11321583
NCBI GeneID
8803
NCBI Accession #
NP_003841
UniProt Primary Accession #
Q9P2R7
UniProt Secondary Accession #
O95194; Q5T9Q4; Q5T9Q6; Q9NV21; Q9NVP7; B2RDE7
UniProt Related Accession #
Q9P2R7
GenBank Nucleotide #
NM_003850
NCBI Official Full Name
succinyl-CoA ligase
NCBI Official Synonym Full Names
succinate-CoA ligase, ADP-forming, beta subunit
NCBI Official Symbol
SUCLA2
NCBI Official Synonym Symbols
A-BETA; MTDPS5; SCS-betaA
UniProt Protein Name
Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
UniProt Synonym Protein Names
ATP-specific succinyl-CoA synthetase subunit beta; Renal carcinoma antigen NY-REN-39; Succinyl-CoA synthetase beta-A chain; SCS-betaA
UniProt Gene Name
SUCLA2
UniProt Synonym Gene Names
SCS-betaA
UniProt Entry Name
SUCB1_HUMAN
NCBI Protein Information
succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial; succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial; ATP-specific succinyl-CoA synthetase subunit beta; ATP-specific succinyl-CoA synthetase, beta subunit; mitochondrial succinyl-CoA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SUCLA2 antibody

 Reference Product  PubMed Publications
 SUCLG1 antibody  >16 publications with SUCLA2 and SUCLG1

Pathways associated with anti-SUCLA2 antibody

 Products by Pathway  Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies  Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
 Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway antibodies  Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate => Oxaloacetate Pathway Diagram
 Citric Acid Cycle (TCA Cycle) Pathway antibodies  Citric Acid Cycle (TCA Cycle) Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-SUCLA2 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >8 publications with SUCLA2 and Liver Diseases
 Atrophy Antibodies  >5 publications with SUCLA2 and Atrophy
 Heart Diseases Antibodies  >2 publications with SUCLA2 and Heart Diseases
 Pain Antibodies  >1 publications with SUCLA2 and Pain
 Kidney Diseases Antibodies  >1 publications with SUCLA2 and Kidney Diseases
 MITOCHONDRIAL DNA DEPLETION SYNDROME 5 (ENCEPHALOMYOPATHIC WITH OR WITHOUT METHYLMALONIC ACIDURIA) Antibodies  >1 publications with SUCLA2 and MITOCHONDRIAL DNA DEPLETION SYNDROME 5 (ENCEPHALOMYOPATHIC WITH OR WITHOUT METHYLMALONIC ACIDURIA)

Organs/Tissues associated with anti-SUCLA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >18 publications with SUCLA2 and Muscle
 Brain Antibodies  >15 publications with SUCLA2 and Brain
 Blood Antibodies  >9 publications with SUCLA2 and Blood
 Liver Antibodies  >7 publications with SUCLA2 and Liver
 Heart Antibodies  >2 publications with SUCLA2 and Heart
 Bone Antibodies  >1 publications with SUCLA2 and Bone
 Skin Antibodies  >1 publications with SUCLA2 and Skin
 Placenta Antibodies  >1 publications with SUCLA2 and Placenta
 Eye Antibodies  >1 publications with SUCLA2 and Eye
 Nerve Antibodies  >1 publications with SUCLA2 and Nerve
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