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SYNGAP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758537

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SYNGAP1, Polyclonal Antibody
Full Product Name
Anti-SYNGAP1 Antibody Picoband
Product Gene Name
anti-SYNGAP1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
1343
OMIM
gene 612621
3D Structure
ModBase 3D Structure for Q96PV0
Immunogen
E coli-derived human SYNGAP1 recombinant protein (Position: R3-R1175). Human SYNGAP1 shares 99% and 99.1% amino acid (aa) sequence identity with mouse and rat SYNGAP1, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Product Categories/Family for anti-SYNGAP1 antibody

Primary Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SYNGAP1 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-SYNGAP1 antibody
WB: 0.25-0.5ug/ml, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-SYNGAP1 antibody

Figure 1. Western blot analysis of SYNGAP1 using anti-SYNGAP1 antibody (MBS1758537).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNGAP1 antigen affinity purified polyclonal antibody (#MBS1758537) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SYNGAP1 at approximately 148 kDa. The expected band size for SYNGAP1 is at 148 kDa.
Rabbit Anti-SYNGAP1 Polyclonal Antibody – Cat# MBS1758537

Flow Cytometry (FC/FACS) of anti-SYNGAP1 antibody

Figure 2. Flow Cytometry analysis of K562 cells using anti-SYNGAP1 antibody (MBS1758537).
Overlay histogram showing K562 cells stained with MBS1758537 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYNGAP1 Antibody (MBS1758537, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-SYNGAP1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758537

Product References and Citations for anti-SYNGAP1 antibody

1. Berryer, M. H., Hamdan, F. F., Klitten, L. L., Moller, R. S., Carmant, L., Schwartzentruber, J., Patry, L., Dobrzeniecka, S., Rochefort, D., Neugnot-Cerioli, M., Lacaille, J.-C., Niu, Z., and 15 others. Mutations in SYNGAP1 cause intellectual disability, autism, and a specific form of epilepsy by inducing haploinsufficiency. Hum. Mutat. 34: 385-394, 2013. 2. Carvill, G. L., Heavin, S. B., Yendle, S. C., McMahon, J. M., O'Roak, B. J., Cook, J., Khan, A., Dorschner, M. O., Weaver, M., Calvert, S., Malone, S., Wallace, G., and 22 others. Targeted resequencing in epileptic encephalopathies identifies de novo mutations in CHD2 and SYNGAP1. Nature Genet. 45: 825-830, 2013. 3. Chen, H.-J., Rojas-Soto, M., Oguni, A., Kennedy, M. B. A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by CaM kinase II. Neuron 20: 895-904, 1998. Note: Erratum: Neuron 33: 151 only, 2002.

Other Notes

Small volumes of anti-SYNGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SYNGAP1 purchase

MBS1758537 is an antibody targeting SYNGAP1. Also known as Anti-SYNGAP1 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SYNGAP1. It may not necessarily be applicable to this product.

NCBI GI #
150421676
NCBI GeneID
8831
NCBI Accession #
Q96PV0.4
UniProt Primary Accession #
Q96PV0
UniProt Secondary Accession #
Q8TCS2; Q9UGE2; A2AB17; A2BEL6; A2BEL7; A8MQC4
UniProt Related Accession #
Q96PV0
NCBI Official Full Name
Ras/Rap GTPase-activating protein SynGAP
NCBI Official Synonym Full Names
synaptic Ras GTPase activating protein 1
NCBI Official Symbol
SYNGAP1
NCBI Official Synonym Symbols
MRD5; RASA1; RASA5; SYNGAP
UniProt Protein Name
Ras/Rap GTPase-activating protein SynGAP
UniProt Synonym Protein Names
Neuronal RasGAP; Synaptic Ras GTPase-activating protein 1; Synaptic Ras-GAP 1
Protein Family
Ras/Rap GTPase-activating protein
UniProt Gene Name
SYNGAP1
UniProt Synonym Gene Names
KIAA1938; Synaptic Ras-GAP 1
UniProt Entry Name
SYGP1_HUMAN
NCBI Protein Information
ras/Rap GTPase-activating protein SynGAP; neuronal RasGAP; Ras GTPase-activating protein SynGAP; synaptic Ras GTPase-activating protein 1; synaptic Ras GTPase activating protein, 135kDa; synaptic Ras GTPase activating protein 1 homolog

NCBI Summary for SYNGAP1

The protein encoded by this gene is a major component of the postsynaptic density (PSD), a group of proteins found associated with NMDA receptors at synapses. The encoded protein is phosphorylated by calmodulin-dependent protein kinase II and dephosphorylated by NMDA receptor activation. Defects in this gene are a cause of mental retardation autosomal dominant type 5 (MRD5). [provided by RefSeq, Dec 2009]
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UniProt Comments for SYNGAP1

SynGAP: an excitatory synapse-specific RasGAP. Member of the NMDAR signaling complex in excitatory synapses it may play a role in NMDAR-dependent control of AMPAR potentiation and synaptic plasticity. ERK activation is up-regulated in neurons from SynGAP knockout mice, whereas P38 MAPK function is depressed. May play a critical role in the regulation of neuronal MAPK signaling, AMPAR membrane trafficking, and excitatory synaptic transmission. Interacts with CAMK2A/CAMK2B and MPDZ. Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. Contains a PH domain and a Ras-GAP domain. Two alternatively spliced isoforms have been described.

Protein type: GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; dendritic shaft

Molecular Function: SH3 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; regulation of MAPKKK cascade; regulation of synapse structure and activity; Ras protein signal transduction; dendrite development; regulation of synaptic plasticity; visual learning; negative regulation of Ras protein signal transduction; negative regulation of axonogenesis; negative regulation of neuron apoptosis; pattern specification process; receptor clustering

Disease: Mental Retardation, Autosomal Dominant 5
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Research Articles on SYNGAP1

1. Reduced cognition in mutant Syngap1 transgenic mice is caused by isolated damage to developing forebrain neurons.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SYNGAP1 antibody

 Reference Product  PubMed Publications
 MPDZ antibody  >3 publications with SYNGAP1 and MPDZ
 DLG4 antibody  >2 publications with SYNGAP1 and DLG4
 GRIN2B antibody  >1 publications with SYNGAP1 and GRIN2B
 CAMK2A antibody  >1 publications with SYNGAP1 and CAMK2A
 SHANK2 antibody  >1 publications with SYNGAP1 and SHANK2
 DDX53 antibody  >1 publications with SYNGAP1 and DDX53
 PTCHD1 antibody  >1 publications with SYNGAP1 and PTCHD1
 DLGAP2 antibody  >1 publications with SYNGAP1 and DLGAP2

Pathways associated with anti-SYNGAP1 antibody

 Products by Pathway  Pathway Diagram
 Ras Signaling Pathway antibodies  Ras Signaling Pathway Diagram

Diseases associated with anti-SYNGAP1 antibody

 Disease Name  Pubmed Publications
 Intellectual Disability Antibodies  >18 publications with SYNGAP1 and Intellectual Disability
 Brain Diseases Antibodies  >11 publications with SYNGAP1 and Brain Diseases
 Epilepsy Antibodies  >9 publications with SYNGAP1 and Epilepsy
 Cognition Disorders Antibodies  >5 publications with SYNGAP1 and Cognition Disorders
 Autistic Disorder Antibodies  >4 publications with SYNGAP1 and Autistic Disorder
 Brain Ischemia Antibodies  >2 publications with SYNGAP1 and Brain Ischemia
 Pain Antibodies  >1 publications with SYNGAP1 and Pain
 Abnormalities, Drug-Induced Antibodies  >1 publications with SYNGAP1 and Abnormalities, Drug-Induced

Organs/Tissues associated with anti-SYNGAP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >38 publications with SYNGAP1 and Brain
 Embryonic Tissue Antibodies  >3 publications with SYNGAP1 and Embryonic Tissue
 Pancreas Antibodies  >1 publications with SYNGAP1 and Pancreas
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