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TK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9502556

MBS9502556 is an antibody targeting TK. Also known as TK (Phospho-Ser13) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TK, Polyclonal Antibody
Full Product Name
TK (Phospho-Ser13) Antibody
Product Gene Name
Product Synonym Gene Name
TK1
Product Synonym Names
KITH; TK-1; TK1; Thymidine kinase; cytosolic
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
1 mg/ml (lot specific)
Specificity
TK (Phospho-Ser13) Antibody detects endogenous levels of TK only when phosphorylated at Ser13.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Molecular Weight
25 kDa
OMIM
K02581 mRNA
3D Structure
ModBase 3D Structure for P04183
Target Modification
Phospho
Modification Sites
H:S13; M:S13; R:S13
Immunogen
The antiserum was produced against synthesized peptide derived from human TK around the phosphorylation site of Ser13.
Immunogen Range
Jan-50
Applications Tested/Suitable for anti-TK antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-TK antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000

Immunohistochemistry (IHC) of anti-TK antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using TK (Phospho-Ser13) Antibody. The picture on the right is blocked with the phospho peptide.
Rabbit Anti-TK Polyclonal Antibody – Cat# MBS9502556

Western Blot (WB) of anti-TK antibody

Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using TK (Phospho-Ser13) Antibody. The lane on the right is blocked with the phospho peptide.
Rabbit Anti-TK Polyclonal Antibody, Western Blot (WB) – Cat# MBS9502556

Other Notes

Small volumes of anti-TK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
thymidine kinase, cytosolic isoform 1
NCBI Official Synonym Full Names
thymidine kinase 1
NCBI Official Symbol
TK1
NCBI Official Synonym Symbols
TK2
UniProt Protein Name
Thymidine kinase, cytosolic
UniProt Gene Name
TK1
NCBI Protein Information
thymidine kinase, cytosolic

NCBI Summary for TK

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]
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UniProt Comments for TK

MiscellaneousTwo forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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