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UCHL1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9231716

Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)Background: UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to… More details

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Product Overview

Product Name
UCHL1, Polyclonal Antibody
Full Product Name
UCHL1 Antibody (C-term)
Product Gene Name
anti-UCHL1 antibody
Product Synonym Names
UCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1
Clonality
Polyclonal
Isotype
Ig
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
24824
OMIM
191342
3D Structure
ModBase 3D Structure for P09936
Antigen Source
Human
Antigen Region
187-214
Antigen Type
Synthetic Peptide
Applications Tested/Suitable for anti-UCHL1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-UCHL1 antibody
WB: 1:1000

Western Blot (WB) of anti-UCHL1 antibody

Western blot analysis of lysates from SH-SY5Y cell line,mouse brain tissue lysate (from left to right), using UCHL1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Rabbit Anti-UCHL1 Polyclonal Antibody – Cat# MBS9231716

Western Blot (WB) of anti-UCHL1 antibody

Western blot analysis of lysates from mouse Neuro-2a,U266,rat PC-12 cell line,mouse brain tissue lysate(from left to right), using UCHL1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
Rabbit Anti-UCHL1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9231716

Western Blot (WB) of anti-UCHL1 antibody

Western blot analysis of lysates from U266,PC-12,NCI-H1299 cell line and mouse brain tissue lysate(from left to right), using UCHL1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Rabbit Anti-UCHL1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9231716

Product References and Citations for anti-UCHL1 antibody

Maraganore, D.M., et al., Mov Disord 18(6):631-636 (2003). Nishikawa, K., et al., Biochem. Biophys. Res. Commun. 304(1):176-183 (2003). Liu, Y., et al., Cell 111(2):209-218 (2002). Caballero, O.L., et al., Oncogene 21(19):3003-3010 (2002). Saigoh, K., et al., Nat. Genet. 23(1):47-51 (1999).

Other Notes

Small volumes of anti-UCHL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UCHL1 purchase

MBS9231716 is an antibody targeting UCHL1. Also known as UCHL1 Antibody (C-term). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UCHL1. It may not necessarily be applicable to this product.

NCBI GI #
21361091
NCBI GeneID
7345
NCBI Accession #
NP_004172.2
UniProt Primary Accession #
P09936
UniProt Secondary Accession #
Q00981; Q6SEG5; Q9R0P9; Q60HC8; P23356
UniProt Related Accession #
P09936
GenBank Nucleotide #
NP_004172.2
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names
Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
Protein Family
Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
UniProt Entry Name
UCHL1_HUMAN
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1

NCBI Summary for UCHL1

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
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UniProt Comments for UCHL1

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; omega peptidase activity; ubiquitin binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; negative regulation of MAP kinase activity; protein deubiquitination; axon transport of mitochondrion; axon target recognition; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant
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Research Articles on UCHL1

1. Results suggest that NR_102714 and its associated protein-coding gene UCHL1 might be involved in the development of chronic obstructive disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UCHL1 antibody

 Reference Product  PubMed Publications
 HSP90AA1 antibody  >2 publications with UCHL1 and HSP90AA1
 CDKN1B antibody  >2 publications with UCHL1 and CDKN1B
 NEDD8 antibody  >1 publications with UCHL1 and NEDD8
 LAMP2 antibody  >1 publications with UCHL1 and LAMP2
 HSPA8 antibody  >1 publications with UCHL1 and HSPA8

Pathways associated with anti-UCHL1 antibody

 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram

Diseases associated with anti-UCHL1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >263 publications with UCHL1 and Neoplasms
 Nervous System Diseases Antibodies  >192 publications with UCHL1 and Nervous System Diseases
 Brain Diseases Antibodies  >142 publications with UCHL1 and Brain Diseases
 Inflammation Antibodies  >46 publications with UCHL1 and Inflammation
 Adenocarcinoma Antibodies  >29 publications with UCHL1 and Adenocarcinoma
 Peripheral Nervous System Diseases Antibodies  >29 publications with UCHL1 and Peripheral Nervous System Diseases
 Cardiovascular Diseases Antibodies  >27 publications with UCHL1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >27 publications with UCHL1 and Disease Models, Animal
 Necrosis Antibodies  >19 publications with UCHL1 and Necrosis
 Nerve Degeneration Antibodies  >18 publications with UCHL1 and Nerve Degeneration

Organs/Tissues associated with anti-UCHL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >173 publications with UCHL1 and Nerve
 Brain Antibodies  >106 publications with UCHL1 and Brain
 Skin Antibodies  >85 publications with UCHL1 and Skin
 Bone Antibodies  >59 publications with UCHL1 and Bone
 Lymph Antibodies  >55 publications with UCHL1 and Lymph
 Lung Antibodies  >36 publications with UCHL1 and Lung
 Mouth Antibodies  >27 publications with UCHL1 and Mouth
 Testis Antibodies  >20 publications with UCHL1 and Testis
 Uterus Antibodies  >19 publications with UCHL1 and Uterus
 Embryonic Tissue Antibodies  >18 publications with UCHL1 and Embryonic Tissue
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