• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

VIM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9703362

VIM encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin encoded by this gene is responsible for maintaining cell shape, integrity of the… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
VIM, Polyclonal Antibody
Full Product Name
VIM Polyclonal Antibody
Product Gene Name
anti-VIM antibody
Product Synonym Names
Rabbit Anti-VIM Polyclonal Antibody; VIM; Vimentin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml (lot specific)
Specificity
VIM Polyclonal Antibody detects endogenous levels of VIM protein.
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
OMIM
193060
3D Structure
ModBase 3D Structure for P08670
Immunogen
Synthesized peptide derived from the C-terminal region of human VIM at AA range: 380-460
Applications Tested/Suitable for anti-VIM antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-VIM antibody
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-VIM antibody

Fig.1. Western Blot analysis of HepG2 cells using VIM Polyclonal Antibody diluted at 1:1000.
Rabbit Anti-VIM Polyclonal Antibody – Cat# MBS9703362

Immunohistochemistry (IHC) of anti-VIM antibody

Fig.2. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue. 1, VIM Polyclonal Antibody was diluted at 1:200 (4 degree,overnight). 2, Sodium citrate pH 6.0 was used for anbitody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min) Negtive control was used by secondary antibody only.).
Rabbit Anti-VIM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9703362

Immunohistochemistry (IHC) of anti-VIM antibody

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue. 1, VIM Polyclonal Antibody was diluted at 1:200 (4 degree,overnight). 2, Sodium citrate pH 6.0 was used for anbitody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min) Negtive control was used by secondary antibody only.).
Rabbit Anti-VIM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9703362

Immunohistochemistry (IHC) of anti-VIM antibody

Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, VIM Polyclonal Antibody was diluted at 1:200 (4 degree,overnight). 2, Sodium citrate pH 6.0 was used for anbitody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min) Negtive control was used by secondary antibody only.
Rabbit Anti-VIM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9703362

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS9703362 is an antibody targeting VIM. Also known as VIM Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Primary Accession #
P08670
UniProt Related Accession #
P08670
GenBank Nucleotide #
NP_003371.2
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Show more

UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30
Show more

Research Articles on VIM

1. Therefore, the present study demonstrated that ZEB1 is a potential biomarker of the tumorigenesis and progression of HCC, and it may regulate transcription of the VIM gene.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-VIM antibody

 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies  Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000