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WEE1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9407093

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates… More details

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Product Overview

Online Datasheet in PDF
Product Name
WEE1, Polyclonal Antibody
Full Product Name
WEE1 (Phospho-Ser642) Antibody
Product Gene Name
anti-WEE1 antibody
Product Synonym Names
WEE1A; WEE1hu; kinase WEE1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration
1.0 mg/ml (lot specific)
Specificity
The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
Preparation and Storage
Store at -20 degree C/1 year
Molecular Weight
49,072 Da
OMIM
193525
3D Structure
ModBase 3D Structure for P30291
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.
Modification
Phospho-Ser642
Target Name
WEE1

Product Categories/Family for anti-WEE1 antibody

Phospho-specific Ab
Applications Tested/Suitable for anti-WEE1 antibody
Western Blot (WB)
Application Notes for anti-WEE1 antibody
Western blotting: 1:500~1:1000

Western Blot (WB) of anti-WEE1 antibody

Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.
Rabbit Anti-WEE1 Polyclonal Antibody – Cat# MBS9407093

Other Notes

Small volumes of anti-WEE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WEE1 purchase

MBS9407093 is an antibody targeting WEE1. Also known as WEE1 (Phospho-Ser642) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WEE1. It may not necessarily be applicable to this product.

NCBI GI #
221307502
NCBI GeneID
7465
NCBI Accession #
NP_001137448.1
UniProt Primary Accession #
P30291
UniProt Secondary Accession #
B3KVE1; D3DQV0
UniProt Related Accession #
P30291
GenBank Nucleotide #
NM_001143976.1
NCBI Official Full Name
wee1-like protein kinase isoform 2
NCBI Official Synonym Full Names
WEE1 G2 checkpoint kinase
NCBI Official Symbol
WEE1
NCBI Official Synonym Symbols
WEE1A; WEE1hu
UniProt Protein Name
Wee1-like protein kinase
UniProt Synonym Protein Names
Wee1A kinase
Protein Family
Mitosis inhibitor protein kinase
UniProt Gene Name
WEE1
UniProt Synonym Gene Names
WEE1hu
UniProt Entry Name
WEE1_HUMAN
NCBI Protein Information
wee1-like protein kinase

NCBI Summary for WEE1

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
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UniProt Comments for WEE1

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: Protein kinase, Other; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: mitosis; peptidyl-tyrosine phosphorylation; establishment of cell polarity; regulation of cell cycle; cell division; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; blood coagulation; G2/M transition of mitotic cell cycle; neurite morphogenesis; G1/S transition of mitotic cell cycle
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Research Articles on WEE1

1. WEE1 is a valid target of the miR-17-92 cluster in leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WEE1 antibody

 Reference Product  PubMed Publications
 CDK1 antibody  >166 publications with WEE1 and CDK1
 CCNB1 antibody  >86 publications with WEE1 and CCNB1
 CDC25C antibody  >69 publications with WEE1 and CDC25C
 CDK2 antibody  >47 publications with WEE1 and CDK2
 PLK1 antibody  >26 publications with WEE1 and PLK1
 CDC25A antibody  >19 publications with WEE1 and CDC25A
 CDC25B antibody  >17 publications with WEE1 and CDC25B
 HSP90AA1 antibody  >1 publications with WEE1 and HSP90AA1

Pathways associated with anti-WEE1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle - G2/M Transition Pathway antibodies  Cell Cycle - G2/M Transition Pathway Diagram
 Cell Cycle - G2/M Transition Pathway antibodies  Cell Cycle - G2/M Transition Pathway Diagram
 Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway antibodies  Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway Diagram
 Cyclin A/B1 Associated Events During G2/M Transition Pathway antibodies  Cyclin A/B1 Associated Events During G2/M Transition Pathway Diagram

Diseases associated with anti-WEE1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >24 publications with WEE1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >16 publications with WEE1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >15 publications with WEE1 and Liver Diseases
 Brain Diseases Antibodies  >13 publications with WEE1 and Brain Diseases
 Carcinoma, Squamous Cell Antibodies  >13 publications with WEE1 and Carcinoma, Squamous Cell
 Liver Neoplasms Antibodies  >10 publications with WEE1 and Liver Neoplasms
 Lung Neoplasms Antibodies  >10 publications with WEE1 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >9 publications with WEE1 and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >8 publications with WEE1 and Cell Transformation, Neoplastic
 Pancreatic Neoplasms Antibodies  >6 publications with WEE1 and Pancreatic Neoplasms

Organs/Tissues associated with anti-WEE1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >26 publications with WEE1 and Liver
 Brain Antibodies  >24 publications with WEE1 and Brain
 Blood Antibodies  >22 publications with WEE1 and Blood
 Lung Antibodies  >17 publications with WEE1 and Lung
 Prostate Antibodies  >16 publications with WEE1 and Prostate
 Kidney Antibodies  >14 publications with WEE1 and Kidney
 Bone Antibodies  >11 publications with WEE1 and Bone
 Ovary Antibodies  >9 publications with WEE1 and Ovary
 Embryonic Tissue Antibodies  >8 publications with WEE1 and Embryonic Tissue
 Vascular Antibodies  >8 publications with WEE1 and Vascular
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