Rabbit Anti-WNK4 Polyclonal Antibody – Cat# MBS9613579

WNK4 Antibody – Rabbit Polyclonal

Name: WNK4 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9613579
Price: 325 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9613579

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride… More details

$325 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

WNK4, Polyclonal Antibody

Full Product Name

WNK4 Antibody

Product Gene Name

anti-WNK4 antibody

Product Synonym Names

PHA 2B; PHA2B; PRKWNK 4; PRKWNK4; Protein kinase lysine deficient 4; Protein kinase lysine-deficient 4; Protein kinase with no lysine 4; Serine/threonine protein kinase WNK4; Serine/threonine-protein kinase WNK4; WNK lysine deficient protein kinase 4; Wnk4; WNK4_HUMAN

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Concentration

1mg/ml (lot specific)

Specificity

WNK4 Antibody detects endogenous levels of total WNK4.
Tissue Specificity: Expressed in kidney, colon and skin.

Preparation and Storage

Store at -20 degree C. Stable for 12 months from date of receipt.

Antibody/Peptide Pairs

WNK4 peptide (MBS9617659) is used for blocking the activity of WNK4 antibody (MBS9613579)

Molecular Weight

Predicted Mol.Wt.: 135kDa (Calculated)

OMIM

145260

3D Structure

ModBase 3D Structure for Q96J92

Supply Chain Verification

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Immunogen

A synthesized peptide derived from human WNK4.

Conjugation

Unconjugated

Fragment

Fab fragment

Post Translational Modifications

Phosphorylated by WNK1 and WNK3.Ubiquitinated by the BCR (KLHL3) complex, leading to its degradation and increased expression of KCNJ1 at the cell surface. Ubiquitinated by the BCR (KLHL2) complex.

Subunit Structure

Interacts with the C-terminal region of KCNJ1 (By similarity). Interacts with WNK1 and WNK3 (By similarity). Interacts with KLHL3.

Similarity

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.

Subcellular Location

Cell junction>Tight junction.
Note: Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-WNK4 antibody

Peptide ELISA (EIA)

Application Notes for anti-WNK4 antibody

Peptide ELISA: 1:20,000-1:40,000

Other Notes

Small volumes of anti-WNK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNK4 purchase

MBS9613579 is an antibody targeting WNK4. Also known as WNK4 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WNK4. It may not necessarily be applicable to this product.

NCBI GeneID

65266

UniProt Primary Accession #

Q96J92

UniProt Protein Name

Serine/threonine-protein kinase WNK4

UniProt Synonym Protein Names

Protein kinase lysine-deficient 4; Protein kinase with no lysine 4

Protein Family

Probable serine/threonine-protein kinase

UniProt Gene Name

WNK4

UniProt Synonym Gene Names

PRKWNK4

UniProt Entry Name

WNK4_HUMAN

UniProt Comments for WNK4

WNK4: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Interacts with the C-terminal region of KCNJ1. Interacts with WNK1 and WNK3. Expressed in kidney, colon and skin. Activation requires autophosphorylation of Ser- 335. Phosphorylation of Ser-331 also promotes increased activity. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; Wnk family

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: tight junction; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; chloride channel inhibitor activity; ATP binding

Biological Process: protein localization; ion homeostasis; regulation of cellular process; ion transport; chloride transport; protein amino acid phosphorylation

Disease: Pseudohypoaldosteronism, Type Iib

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.