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WNT5A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6363727

MBS6363727 is an antibody targeting WNT5A. Also known as WNT5A, ID (WNT5A, Protein Wnt-5a) (MaxLight 550). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WNT5A, Polyclonal Antibody
Full Product Name
WNT5A, ID (WNT5A, Protein Wnt-5a) (MaxLight 550)
Product Gene Name
Product Synonym Names
WNT5A; Protein Wnt-5a
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human WNT5A. Species Crossreactivity: mouse, rat
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
40,887 Da
OMIM
164975
3D Structure
ModBase 3D Structure for P41221
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 185-213 in the central region of human WNT5A, Swiss/UniProt Accession: P41221
Conjugate
MaxLight550
Note
Preservative Free

Product Categories/Family for anti-WNT5A antibody

Applications Tested/Suitable for anti-WNT5A antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-WNT5A antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-WNT5A antibody

Western Blot of Hela cell line lysates (35ug/lane) usingMBS6002362. This demonstrates that MBS6002362 detected the WNT5A protein (arrow).
Rabbit Anti-WNT5A Polyclonal Antibody – Cat# MBS6363727

Testing Data of anti-WNT5A antibody

All lanes: MBS6002362 (1:500) Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-WNT5A Polyclonal Antibody, Testing Data – Cat# MBS6363727

Immunohistochemistry (IHC) of anti-WNT5A antibody

Immunohistochemistry of formalin fixed and paraffin embedded human lung carcinoma using MBS6002362 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS6002362 for immunohistochemistry.
Rabbit Anti-WNT5A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6363727

Flow Cytometry (FC/FACS) of anti-WNT5A antibody

Flow Cytometric analysis of Hela cells using MBS6002362 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-WNT5A Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6363727

Product References and Citations for anti-WNT5A antibody

He, X., et al. Science 275(5306):1652-1654(1997). Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995) Smolich, B.D., et al. Mol. Biol. Cell 4(12):1267-1275(1993) Clark, C.C., et al. Genomics 18(2):249-260(1993).

Other Notes

Small volumes of anti-WNT5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WNT5A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein Wnt-5a isoform 1
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 5A
NCBI Official Symbol
WNT5A
NCBI Official Synonym Symbols
hWNT5A
UniProt Protein Name
Protein Wnt-5a
Protein Family
UniProt Gene Name
WNT5A
UniProt Entry Name
WNT5A_HUMAN
NCBI Protein Information
protein Wnt-5a; WNT-5A protein

NCBI Summary for WNT5A

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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