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Caspase 1 (CASP1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002145
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MBS2002145 is an antibody targeting CASP1. Also known as Polyclonal Antibody to Caspase 1 (CASP1). Reactive in Human, Pig. Host: Rabbit. More details

$230 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Caspase 1 (CASP1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Caspase 1 (CASP1)
Product Gene Name
anti-CASP1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Pig
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: CASP1 (MBS2002145)
Biotin Conjugated Antibody: CASP1 (MBS2005384)
Matching Pairs
Unconjugated Antibody: CASP1 (MBS2002145)
Immunogen: CASP1 (MBS2009994)
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3D Structure
ModBase 3D Structure for Q9N2I1
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human
Immunogen
Recombinant CASP1 (Asn120~Asp297) expressed in E.coli (MBS2009994)

Product Categories/Family for anti-CASP1 antibody

Knock-Out Validation Antibody
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-CASP1 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-CASP1 antibody
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-CASP1 antibody

Sample: Recombinant CASP1, Porcine.
Rabbit Anti-CASP1 Polyclonal Antibody – Cat# MBS2002145

Western Blot (WB) of anti-CASP1 antibody

Sample:
Lane1: Porcine Spleen lysate;
Lane2: Porcine Lung lysate;
Lane3: Raji cell lysate
Primary Ab: 1ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-CASP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002145

Knockout Verification of anti-CASP1 antibody

Knockout Varification:
Lane 1: Wild-type Raji cell lysate;
Lane 2: CASP1 knockout Raji cell lysate;
Predicted MW: 10,30,35,43,45kDa
Observed MW: 42kDa
Primary Ab: 5ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-CASP1 Polyclonal Antibody, Knockout Verification – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Samples: Porcine Cardiac Muscle Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Samples: Porcine Kidney Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Samples: Porcine Spleen Tissue;
Primary Ab: 10ug/ml Rabbit Anti- Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Samples: Porcine Pancreas Tissue;
Primary Ab: 10ug/ml Rabbit Anti- Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Samples: Porcine Small intestine Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Sample: Human Placenta Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunohistochemistry (IHC) of anti-CASP1 antibody

DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-CASP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002145

Immunofluorescence (IF) of anti-CASP1 antibody

FITC staining on IF;
Sample: Human U2OS cell;
Primary Ab: 30ug/ml Rabbit Anti-Porcine CASP1 Antibody
Second Ab: 2ug/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086046)
Rabbit Anti-CASP1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS2002145

Other Notes

Small volumes of anti-CASP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CASP1. It may not necessarily be applicable to this product.

NCBI GI #
47523412
NCBI GeneID
397319
NCBI Accession #
NP_999327.1
UniProt Primary Accession #
Q9N2I1
UniProt Related Accession #
Q9N2I1
GenBank Nucleotide #
NM_214162.1
NCBI Official Full Name
caspase-1
NCBI Official Symbol
CASP1
UniProt Protein Name
Caspase-1
UniProt Synonym Protein Names
Interleukin-1 beta convertase; IL-1BC
UniProt Gene Name
CASP1
UniProt Synonym Gene Names
IL1BC; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
NCBI Protein Information
caspase-1

UniProt Comments for CASP1

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of MB21D1/cGAS, rendering it inactive.
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Research Articles on CASP1

1. mmediately after hypoxia C8 and C1 follow a similar pattern of increase while long term this appears to dissociate

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CASP1 antibody

 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram

Diseases associated with anti-CASP1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >780 publications with CASP1 and Inflammation
 Necrosis Antibodies  >504 publications with CASP1 and Necrosis
 Neoplasms Antibodies  >383 publications with CASP1 and Neoplasms
 Nervous System Diseases Antibodies  >297 publications with CASP1 and Nervous System Diseases
 Brain Diseases Antibodies  >228 publications with CASP1 and Brain Diseases
 Drug Toxicity Antibodies  >180 publications with CASP1 and Drug Toxicity
 Gastrointestinal Diseases Antibodies  >114 publications with CASP1 and Gastrointestinal Diseases
 Carcinoma Antibodies  >101 publications with CASP1 and Carcinoma
 Liver Diseases Antibodies  >78 publications with CASP1 and Liver Diseases
 Brain Ischemia Antibodies  >72 publications with CASP1 and Brain Ischemia

Organs/Tissues associated with anti-CASP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >755 publications with CASP1 and Blood
 Lung Antibodies  >132 publications with CASP1 and Lung
 Skin Antibodies  >109 publications with CASP1 and Skin
 Intestine Antibodies  >79 publications with CASP1 and Intestine
 Thymus Antibodies  >29 publications with CASP1 and Thymus
 Uterus Antibodies  >14 publications with CASP1 and Uterus
 Aorta Antibodies  >9 publications with CASP1 and Aorta
 Oviduct Antibodies  >7 publications with CASP1 and Oviduct
 Bladder Antibodies  >4 publications with CASP1 and Bladder
 Esophagus Antibodies  >3 publications with CASP1 and Esophagus
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