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RFC4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9126290
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The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RFC4, Polyclonal Antibody
Full Product Name
RFC4 Polyclonal Antibody
Product Gene Name
anti-RFC4 antibody
Product Synonym Names
A1; RFC37
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
363
OMIM
102577
3D Structure
ModBase 3D Structure for P35249
Species
Human
Immunogen
Recombinant protein of human RFC4
Calculated Molecular Weight
39kDa

Product Categories/Family for anti-RFC4 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-RFC4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RFC4 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-RFC4 antibody

Western blot analysis of extracts of various cell lines, using RFC4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Rabbit Anti-RFC4 Polyclonal Antibody – Cat# MBS9126290

Immunohistochemistry (IHC) of anti-RFC4 antibody

Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-RFC4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9126290

Immunohistochemistry (IHC) of anti-RFC4 antibody

Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-RFC4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9126290

Immunohistochemistry (IHC) of anti-RFC4 antibody

Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-RFC4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9126290

Immunofluorescence (IF) of anti-RFC4 antibody

Immunofluorescence analysis of U2OS cells using RFC4 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-RFC4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9126290

Immunofluorescence (IF) of anti-RFC4 antibody

Immunofluorescence analysis of U2OS cells using RFC4 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-RFC4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9126290

Immunoprecipitation (IP) of anti-RFC4 antibody

Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody. Western blot was performed from the immunoprecipitate using RFC4 antibody at a dilition of 1:1000.
Rabbit Anti-RFC4 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS9126290

Other Notes

Small volumes of anti-RFC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFC4 purchase

MBS9126290 is an antibody targeting RFC4. Also known as RFC4 Polyclonal Antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RFC4. It may not necessarily be applicable to this product.

NCBI GI #
1703052
NCBI GeneID
5984
NCBI Accession #
P35249.2
UniProt Primary Accession #
P35249
UniProt Secondary Accession #
Q6FHX7; B4DM41; D3DNV2
UniProt Related Accession #
P35249
NCBI Official Full Name
Replication factor C subunit 4
NCBI Official Synonym Full Names
replication factor C (activator 1) 4, 37kDa
NCBI Official Symbol
RFC4
NCBI Official Synonym Symbols
A1; RFC37
UniProt Protein Name
Replication factor C subunit 4
UniProt Synonym Protein Names
Activator 1 37 kDa subunit; A1 37 kDa subunit; Activator 1 subunit 4; Replication factor C 37 kDa subunit; RF-C 37 kDa subunit; RFC37
Protein Family
Probable replication factor
UniProt Gene Name
RFC4
UniProt Synonym Gene Names
A1 37 kDa subunit; RF-C 37 kDa subunit; RFC37
UniProt Entry Name
RFC4_HUMAN
NCBI Protein Information
replication factor C subunit 4; A1 37 kDa subunit; RFC 37 kDa subunit; RF-C 37 kDa subunit; activator 1 subunit 4; activator 1 37 kDa subunit; replication factor C 37 kDa subunit

UniProt Comments for RFC4

RFC4: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. Belongs to the activator 1 small subunits family.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: nucleoplasm; DNA replication factor C complex; cytoplasm; nucleus

Molecular Function: protein binding; ATP binding; DNA clamp loader activity

Biological Process: telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; DNA strand elongation during DNA replication; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA repair; telomere maintenance
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Research Articles on RFC4

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RFC4 antibody

 Reference Product  PubMed Publications
 RFC3 antibody  >27 publications with RFC4 and RFC3
 PCNA antibody  >27 publications with RFC4 and PCNA
 RFC2 antibody  >26 publications with RFC4 and RFC2
 RFC5 antibody  >18 publications with RFC4 and RFC5
 RFC1 antibody  >9 publications with RFC4 and RFC1
 RAD17 antibody  >4 publications with RFC4 and RAD17
 CDC6 antibody  >1 publications with RFC4 and CDC6
 PRIM1 antibody  >1 publications with RFC4 and PRIM1

Pathways associated with anti-RFC4 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-RFC4 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >9 publications with RFC4 and Carcinoma
 Adenocarcinoma Antibodies  >5 publications with RFC4 and Adenocarcinoma
 Liver Diseases Antibodies  >4 publications with RFC4 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with RFC4 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >4 publications with RFC4 and Liver Neoplasms
 Carcinoma, Squamous Cell Antibodies  >2 publications with RFC4 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >2 publications with RFC4 and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with RFC4 and Neoplasm Metastasis
 Cell Transformation, Neoplastic Antibodies  >2 publications with RFC4 and Cell Transformation, Neoplastic
 Uterine Cervical Neoplasms Antibodies  >2 publications with RFC4 and Uterine Cervical Neoplasms

Organs/Tissues associated with anti-RFC4 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >5 publications with RFC4 and Liver
 Blood Antibodies  >3 publications with RFC4 and Blood
 Cervix Antibodies  >2 publications with RFC4 and Cervix
 Lung Antibodies  >2 publications with RFC4 and Lung
 Prostate Antibodies  >2 publications with RFC4 and Prostate
 Thymus Antibodies  >2 publications with RFC4 and Thymus
 Pancreas Antibodies  >2 publications with RFC4 and Pancreas
 Eye Antibodies  >1 publications with RFC4 and Eye
 Lymph Node Antibodies  >1 publications with RFC4 and Lymph Node
 Mouth Antibodies  >1 publications with RFC4 and Mouth
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