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SIRT5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6270117

MBS6270117 is an antibody targeting SIRT5. Also known as SIRT5 (NAD-dependent Deacetylase Sirtuin 5, SIR2-like Protein 5, SIR2L5) (MaxLight 490). Reactive in Human, Rat. Host: Rabbit. More details

$1335 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SIRT5, Polyclonal Antibody
Full Product Name
SIRT5 (NAD-dependent Deacetylase Sirtuin 5, SIR2-like Protein 5, SIR2L5) (MaxLight 490)
Product Gene Name
anti-SIRT5 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human SIRT5. Species Crossreactivity: rat.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
21,689 Da
OMIM
604483
3D Structure
ModBase 3D Structure for Q9NXA8
Immunogen
Synthetic linear peptide corresponding to human SIRT5 (KLH). Species sequence homology: chimpanzee and rhesus macaque (100%).
Conjugate
MaxLight490
Positive Control
HeLa Cell Lysate
Note
Preservative Free

Product Categories/Family for anti-SIRT5 antibody

Antibodies; Sirtuins
Applications Tested/Suitable for anti-SIRT5 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SIRT5 antibody
IHC: 1:500
WB: 0.5ug/ml
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-SIRT5 antibody

Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded convoluted tubules of normal rat kidney tissue (Fig 1 and 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Sirt5. Reactivity was detected using the IHC-Select Detection Kit. Positive granular cutoplasmic staining was observed in convoluted tubules of normal rat kidney tissue.
Rabbit Anti-SIRT5 Polyclonal Antibody – Cat# MBS6270117

Western Blot (WB) of anti-SIRT5 antibody

Western Blotting Analysis: Representative lot data. HeLa cell lysate was probed with Sirt5 (0.5ug/ml). Proteins were visualized using a Donkey Rabbit IgG secondary antibodies conjugated to HRP and a chemiluminescence detection system. Arrow indicates Sirt5 (~34kD).
Rabbit Anti-SIRT5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6270117

Product References and Citations for anti-SIRT5 antibody

1. Matsushita, N., et al. (2011). Genes Cells. 16(2):190-202. 2. Nakagawa, T. and Guarente, L. (2009). Aging (Albany NY). 1(6):578-581. 3. Mahlknecht, U., et al. (2006). Cytogenet Genome Res. 112(3-4):208-212.

Other Notes

Small volumes of anti-SIRT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SIRT5. It may not necessarily be applicable to this product.

NCBI GI #
6912664
NCBI GeneID
23408
NCBI Accession #
NP_036373
UniProt Primary Accession #
Q9NXA8
UniProt Secondary Accession #
Q5T294; Q5T295; Q9Y6E6; B4DFM4; B4DYJ5; F5H5Z9
UniProt Related Accession #
Q9NXA8
GenBank Nucleotide #
NM_012241.4
NCBI Official Full Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial isoform 1
NCBI Official Synonym Full Names
sirtuin 5
NCBI Official Symbol
SIRT5
NCBI Official Synonym Symbols
SIR2L5
UniProt Protein Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Synonym Protein Names
Regulatory protein SIR2 homolog 5; SIR2-like protein 5
Protein Family
NAD-dependent protein deacylase sirtuin
UniProt Gene Name
SIRT5
UniProt Synonym Gene Names
SIR2L5
UniProt Entry Name
SIR5_HUMAN
NCBI Protein Information
NAD-dependent protein deacylase sirtuin-5, mitochondrial; SIRT5; sir2-like 5; sirtuin type 5; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; NAD-dependent lysine demalonylase and desuccinylase si

NCBI Summary for SIRT5

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]
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Research Articles on SIRT5

1. Expression of SIRT5 in the entorhinal cortex, hippocampus, and white matter increases during the progression of Alzheimer disease. It is seen in activated microglia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SIRT5 antibody

 Reference Product  PubMed Publications
 SIRT3 antibody  >59 publications with SIRT5 and SIRT3
 CPS1 antibody  >5 publications with SIRT5 and CPS1
 HDAC9 antibody  >2 publications with SIRT5 and HDAC9

Pathways associated with anti-SIRT5 antibody

 Products by Pathway  Pathway Diagram
 Signaling Events Mediated By HDAC Class I Pathway antibodies  Signaling Events Mediated By HDAC Class I Pathway Diagram

Diseases associated with anti-SIRT5 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >9 publications with SIRT5 and Death
 Fatty Liver Antibodies  >3 publications with SIRT5 and Fatty Liver
 Liver Diseases Antibodies  >2 publications with SIRT5 and Liver Diseases
 Hyperglycemia Antibodies  >2 publications with SIRT5 and Hyperglycemia
 Carcinoma, Hepatocellular Antibodies  >2 publications with SIRT5 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >1 publications with SIRT5 and Inflammation
 Adenocarcinoma Antibodies  >1 publications with SIRT5 and Adenocarcinoma
 Heart Diseases Antibodies  >1 publications with SIRT5 and Heart Diseases
 Abnormalities, Drug-Induced Antibodies  >1 publications with SIRT5 and Abnormalities, Drug-Induced
 Kidney Diseases Antibodies  >1 publications with SIRT5 and Kidney Diseases

Organs/Tissues associated with anti-SIRT5 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >16 publications with SIRT5 and Liver
 Brain Antibodies  >9 publications with SIRT5 and Brain
 Heart Antibodies  >6 publications with SIRT5 and Heart
 Blood Antibodies  >5 publications with SIRT5 and Blood
 Connective Tissue Antibodies  >3 publications with SIRT5 and Connective Tissue
 Lung Antibodies  >3 publications with SIRT5 and Lung
 Adipose Tissue Antibodies  >3 publications with SIRT5 and Adipose Tissue
 Pancreas Antibodies  >2 publications with SIRT5 and Pancreas
 Intestine Antibodies  >1 publications with SIRT5 and Intestine
 Eye Antibodies  >1 publications with SIRT5 and Eye
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