• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Thrombomodulin/THBD (THBD) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758179

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thrombomodulin/THBD, Polyclonal Antibody
Full Product Name
Anti-Thrombomodulin/THBD Antibody Picoband
Product Gene Name
anti-THBD antibody
Product Synonym Names
Heparanase; WEE1 G2 checkpoint kinase; Wee1-like protein kinase; WEE1hu; Wee1A kinase; WEE1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
PredominantlyExpressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
60,329 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (23026270..23030301, complement). Location: 20p11.2
OMIM
188040
3D Structure
ModBase 3D Structure for P07204
Immunogen
E coli-derived human Thrombomodulin/THBD recombinant protein (Position: Q29-L575).
Subcellular Localization
Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Product Categories/Family for anti-THBD antibody

Primary Antibodies; Cell Biology; Cell Cycle; Epigenetics and Nuclear Signaling; Kinases/Phosphatases
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-THBD antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-THBD antibody
WB: 0.25-0.5ug/ml, Human, Rat
IHC(Paraffin-embedded Section): 2-5ug/ml, Human
ICC/IF: 5ug/ml, Human
IP: 0.5-2ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-THBD antibody

Figure 1. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (MBS1758179).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (#MBS1758179) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
Rabbit Anti-THBD Polyclonal Antibody – Cat# MBS1758179

Immunohistochemistry (IHC) of anti-THBD antibody

Figure 2. IHC analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (MBS1758179).
Thrombomodulin/THBD was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Thrombomodulin/THBD Antibody (MBS1758179) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-THBD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758179

Immunohistochemistry (IHC) of anti-THBD antibody

Figure 3. IHC analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (MBS1758179).
Thrombomodulin/THBD was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Thrombomodulin/THBD Antibody (MBS1758179) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-THBD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758179

Immunofluorescence (IF) of anti-THBD antibody

Figure 4. IF analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (MBS1758179) and anti-Tubulin Alpha antibody (M03989-3).
Thrombomodulin/THBD was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-Thrombomodulin/THBD Antibody (MBS1758179) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-THBD Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758179

Flow Cytometry (FC/FACS) of anti-THBD antibody

Figure 5. Flow Cytometry analysis of THP-1 cells using anti-Thrombomodulin/THBD antibody (MBS1758179).
Overlay histogram showing THP-1 cells stained with MBS1758179 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thrombomodulin/THBD Antibody (MBS1758179, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-THBD Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758179

Immunoprecipitation (IP) of anti-THBD antibody

Figure 6. Immunoprecipitating Thrombomodulin/THBD in A431 whole cell lysate .
Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (MBS1758179).
Lane 1: A431 whole cell lysates (30ug)
Lane 2: Rabbit control IgG instead of anti-Thrombomodulin/THBD antibody in A431 whole cell lysate.
Lane 3: anti-Thrombomodulin/THBD antibody (2ug) + A431 whole cell lysate (500ug)
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (MBS1758179) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (#MBS176703). The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
Rabbit Anti-THBD Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS1758179

Product References and Citations for anti-THBD antibody

1. Anastasiou, G., Gialeraki, A., Merkouri, E., Politou, M., Travlou, A. Thrombomodulin as a regulator of the anticoagulant pathway: implication in the development of thrombosis. Blood Coagul. Fibrinolysis 23: 1-10, 2012. 2. Cole, J. W., Roberts, S. C., Gallagher, M., Giles, W. H., Mitchell, B. D., Steinberg, K. K., Wozniak, M. A., Macko, R. F., Reinhart, L. J., Kittner, S. J. Thrombomodulin ala455val polymorphism and the risk of cerebral infarction in a biracial population: the Stroke Prevention in Young Women Study. BMC Neurol. 4: 21-27, 2004. 3. Delvaeye, M., Noris, M., De Vriese, A., Esmon, C. T., Esmon, N. L., Ferrell, G., Del-Favero, J., Plaisance, S., Claes, B., Lambrechts, D., Zoja, C., Remuzzi, G., Conway, E. M. Thrombomodulin mutations in atypical hemolytic-uremic syndrome. New Eng. J. Med. 361: 345-357, 2009.

Other Notes

Small volumes of anti-THBD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for THBD purchase

MBS1758179 is an antibody targeting THBD. Also known as Anti-Thrombomodulin/THBD Antibody Picoband. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for THBD. It may not necessarily be applicable to this product.

NCBI GI #
4507483
NCBI GeneID
7056
NCBI Accession #
NP_000352.1
UniProt Primary Accession #
P07204
UniProt Secondary Accession #
Q8IV29; Q9UC32
UniProt Related Accession #
P07204
GenBank Nucleotide #
NM_000361.2
NCBI Official Full Name
thrombomodulin
NCBI Official Synonym Full Names
thrombomodulin
NCBI Official Symbol
THBD
NCBI Official Synonym Symbols
TM; THRM; AHUS6; BDCA3; CD141; THPH12
UniProt Protein Name
Thrombomodulin
UniProt Synonym Protein Names
Fetomodulin
Protein Family
Thrombomodulin
UniProt Gene Name
THBD
UniProt Synonym Gene Names
THRM; TM
UniProt Entry Name
TRBM_HUMAN
NCBI Protein Information
thrombomodulin; fetomodulin; CD141 antigen

NCBI Summary for THBD

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for THBD

thrombomodulin: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THPH12). A hemostatic disorder characterized by a tendency to thrombosis. Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p11.2

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; calcium ion binding; receptor activity

Biological Process: negative regulation of fibrinolysis; response to cAMP; negative regulation of blood coagulation; response to lipopolysaccharide; female pregnancy; signal transduction; blood coagulation; leukocyte migration; response to X-ray

Disease: Thrombophilia Due To Thrombomodulin Defect; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 6
Show more

Research Articles on THBD

1. FFAs inhibit TM-EPCR-Protein C system in endothelial cells through activating JNK signaling, which may be a mechanism for the prothrombotic state in metabolic syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-THBD antibody

 Reference Product  PubMed Publications
 PROC antibody  >2 publications with THBD and PROC

Pathways associated with anti-THBD antibody

 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway antibodies  Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway Diagram

Diseases associated with anti-THBD antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >31 publications with THBD and Cardiovascular Diseases
 Thrombosis Antibodies  >20 publications with THBD and Thrombosis
 Inflammation Antibodies  >19 publications with THBD and Inflammation
 Neoplasms Antibodies  >18 publications with THBD and Neoplasms
 Kidney Diseases Antibodies  >14 publications with THBD and Kidney Diseases
 Thrombophilia Antibodies  >13 publications with THBD and Thrombophilia
 Disseminated Intravascular Coagulation Antibodies  >10 publications with THBD and Disseminated Intravascular Coagulation
 Heart Diseases Antibodies  >8 publications with THBD and Heart Diseases
 Atypical hemolytic uremic syndrome Antibodies  >7 publications with THBD and Atypical hemolytic uremic syndrome
 Nervous System Diseases Antibodies  >6 publications with THBD and Nervous System Diseases

Organs/Tissues associated with anti-THBD antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >63 publications with THBD and Blood
 Vascular Antibodies  >28 publications with THBD and Vascular
 Liver Antibodies  >9 publications with THBD and Liver
 Kidney Antibodies  >9 publications with THBD and Kidney
 Lung Antibodies  >8 publications with THBD and Lung
 Bone Antibodies  >7 publications with THBD and Bone
 Brain Antibodies  >6 publications with THBD and Brain
 Embryonic Tissue Antibodies  >6 publications with THBD and Embryonic Tissue
 Stomach Antibodies  >5 publications with THBD and Stomach
 Placenta Antibodies  >4 publications with THBD and Placenta
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000