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Sialoprotein, Bone (IBSP) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625963

Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is… More details

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Product Overview

Online Datasheet in PDF
Product Name
Sialoprotein, Bone (IBSP), Polyclonal Antibody
Full Product Name
Sialoprotein, Bone (BSP)
Product Gene Name
Product Synonym Names
Anti -Sialoprotein, Bone (BSP)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as a lyophilized powder. Reconstitute with 20ul ddH2O.
Purity/Purification
Serum
Serum
Specificity
Recognizes human bone sialoprotein. Species Crossreactivity: rat. No cross reactivity with human Osteonectin or human Osteopontin.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
35,148 Da
Chromosome Location
Chromosome: 4; NC_000004.11 (88720702..88733601). Location: 4q21.1
OMIM
147563
3D Structure
ModBase 3D Structure for P21815
Immunogen
Human bone sialoprotein (bone extract)
Applications Tested/Suitable for anti-IBSP antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Radioimmunoassay (RIA)
Application Notes for anti-IBSP antibody
Suitable for use in RIA, ELISA, Western Blot, Immunohistochemistry.
Dilution: Immunohistochemistry: paraffin sections

Other Notes

Small volumes of anti-IBSP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IBSP purchase

MBS625963 is an antibody targeting IBSP. Also known as Sialoprotein, Bone (BSP). Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IBSP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
sialoprotein
NCBI Official Synonym Full Names
integrin-binding sialoprotein
NCBI Official Symbol
IBSP
NCBI Official Synonym Symbols
BSP; BNSP; SP-II; BSP-II
UniProt Protein Name
Bone sialoprotein 2
UniProt Synonym Protein Names
Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein
Protein Family
UniProt Gene Name
IBSP
UniProt Synonym Gene Names
BNSP
UniProt Entry Name
SIAL_HUMAN
NCBI Protein Information
bone sialoprotein 2; BSP II; OTTHUMP00000161175; bone sialoprotein II; cell-binding sialoprotein

NCBI Summary for IBSP

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq]
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UniProt Comments for IBSP

Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: extracellular space; membrane; extracellular region

Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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