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Jagged1 (JAG1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611894

The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Jagged-1 (JAG1) is a… More details

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Product Overview

Online Datasheet in PDF
Product Name
Jagged1 (JAG1), Polyclonal Antibody
Full Product Name
Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille syndrome protein)
Product Gene Name
anti-JAG1 antibody
Product Synonym Names
Anti -Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille syndrome protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Jagged1 (JAG1). A band at ~150kD corresponding to Jagged-1 in mouse liver whole cell lysates and a ~75kD band in human brain and kidney lysates. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 20; NC_000020.10 (10618332..10654694, complement). Location: 20p12.1-p11.23
OMIM
118450
3D Structure
ModBase 3D Structure for P78504
Immunogen
Synthetic peptide corresponding to aa110-125 of human Jagged-1 protein.

Product Categories/Family for anti-JAG1 antibody

Antibodies; Abs to Notch Proteins
Applications Tested/Suitable for anti-JAG1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-JAG1 antibody
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorescence.

Western Blot (WB) of anti-JAG1 antibody

Western blot using MBS611894 shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains ~ 20ug of lysate. MBS611894 was used at a 1:250 dilution. The membrane was washed and reacted with IgG (HRP) Pab Gt xRb Exposure time was 1 min. Predicted molecular weight is 134kD.
Rabbit Anti-JAG1 Polyclonal Antibody – Cat# MBS611894

Immunofluorescence (IF) of anti-JAG1 antibody

Immunofluorescence Microscopy using MBS611894 on human corneal epithelial cells. MBS611894 was used at a 1:250 dilution. The Jagged1 (green staining) is local-ized to the cytoplasm and is consistentwith reports in the literature. The nucleus is stained with Bis benzimide (blue).
Rabbit Anti-JAG1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS611894

Immunohistochemistry (IHC) of anti-JAG1 antibody

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using MBS611894. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:50 dilution of MBS611894 was used.
Rabbit Anti-JAG1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS611894

Other Notes

Small volumes of anti-JAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JAG1 purchase

MBS611894 is an antibody targeting JAG1. Also known as Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille syndrome protein). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for JAG1. It may not necessarily be applicable to this product.

NCBI GI #
2228793
NCBI GeneID
182
UniProt Primary Accession #
P78504
UniProt Secondary Accession #
O14902; O15122; Q15816; A0AV43
UniProt Related Accession #
P78504; Q4KMR2; Q99740
NCBI Official Full Name
Jagged1
NCBI Official Synonym Full Names
jagged 1
NCBI Official Symbol
JAG1
NCBI Official Synonym Symbols
AGS; AHD; AWS; HJ1; CD339; JAGL1; MGC104644
UniProt Protein Name
Protein jagged-1
Protein Family
Protein jagged
UniProt Entry Name
JAG1_HUMAN
NCBI Protein Information
protein jagged-1; OTTHUMP00000030278

NCBI Summary for JAG1

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq]
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UniProt Comments for JAG1

JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12.1-p11.23

Cellular Component: membrane; apical part of cell; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; calcium ion binding; Notch binding; structural molecule activity

Biological Process: nervous system development; endothelial cell differentiation; Notch signaling pathway; positive regulation of myeloid cell differentiation; T cell mediated immunity; Notch receptor processing; auditory receptor cell differentiation; cell fate determination; negative regulation of fat cell differentiation; regulation of cell migration; regulation of cell proliferation; myoblast differentiation; keratinocyte differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; response to muramyl dipeptide; morphogenesis of an epithelial sheet; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; blood vessel remodeling; angiogenesis; positive regulation of Notch signaling pathway

Disease: Tetralogy Of Fallot; Alagille Syndrome 1
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Research Articles on JAG1

1. the interaction between the PDZ domain of afadin (AF6_PDZ) and a series of polypeptides comprising the PDZ-binding motif, was studied.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-JAG1 antibody

 Reference Product  PubMed Publications
 NOTCH1 antibody  >39 publications with JAG1 and NOTCH1
 NOTCH2 antibody  >24 publications with JAG1 and NOTCH2
 NOTCH3 antibody  >14 publications with JAG1 and NOTCH3
 MFNG antibody  >3 publications with JAG1 and MFNG
 JUN antibody  >1 publications with JAG1 and JUN
 FOS antibody  >1 publications with JAG1 and FOS

Pathways associated with anti-JAG1 antibody

 Products by Pathway  Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Notch Receptor Binds With A Ligand Pathway antibodies  Notch Receptor Binds With A Ligand Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Receptor-ligand Binding Initiates The Second Proteolytic Cleavage Of Notch Receptor Pathway antibodies  Receptor-ligand Binding Initiates The Second Proteolytic Cleavage Of Notch Receptor Pathway Diagram
 Signaling By Notch Pathway antibodies  Signaling By Notch Pathway Diagram

Diseases associated with anti-JAG1 antibody

 Disease Name  Pubmed Publications
 Alagille Syndrome Antibodies  >101 publications with JAG1 and Alagille Syndrome
 Congenital Abnormalities Antibodies  >97 publications with JAG1 and Congenital Abnormalities
 Cardiovascular Diseases Antibodies  >95 publications with JAG1 and Cardiovascular Diseases
 Heart Defects, Congenital Antibodies  >93 publications with JAG1 and Heart Defects, Congenital
 Cholestasis Antibodies  >87 publications with JAG1 and Cholestasis
 Neoplasms Antibodies  >31 publications with JAG1 and Neoplasms
 Heart Diseases Antibodies  >21 publications with JAG1 and Heart Diseases
 Chromosome Aberrations Antibodies  >14 publications with JAG1 and Chromosome Aberrations
 Eye Abnormalities Antibodies  >13 publications with JAG1 and Eye Abnormalities
 Tetralogy of Fallot Antibodies  >12 publications with JAG1 and Tetralogy of Fallot

Organs/Tissues associated with anti-JAG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >57 publications with JAG1 and Liver
 Heart Antibodies  >41 publications with JAG1 and Heart
 Vascular Antibodies  >29 publications with JAG1 and Vascular
 Eye Antibodies  >25 publications with JAG1 and Eye
 Blood Antibodies  >25 publications with JAG1 and Blood
 Embryonic Tissue Antibodies  >20 publications with JAG1 and Embryonic Tissue
 Ear Antibodies  >19 publications with JAG1 and Ear
 Kidney Antibodies  >15 publications with JAG1 and Kidney
 Brain Antibodies  >13 publications with JAG1 and Brain
 Muscle Antibodies  >10 publications with JAG1 and Muscle
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