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B7-1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695289

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mB7-1. Anti-Murine B7-1 specific antibody was purified by affinity chromatography and then biotinylated. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
B7-1, Polyclonal Antibody
Full Product Name
Biotinylated Anti-mB7-1
Product Gene Name
anti-B7-1 antibody
Product Synonym Names
Biotinylated Anti-Murine B7-1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Biotin
Preparation and Storage
At -20 degree C
Molecular Weight
32,692 Da
Applications Tested/Suitable for anti-B7-1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-B7-1 antibody
Direct Detection: To detect mB7-1 by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mB7-1.

Sandwich ELISA: To detect mB7-1 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine B7-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mB7-1.

Western Blot: To detect mB7-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mB7-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-B7-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B7-1 purchase

MBS695289 is an antibody targeting B7-1. Also known as Biotinylated Anti-mB7-1. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for B7-1. It may not necessarily be applicable to this product.

NCBI GI #
507873
NCBI GeneID
25408
NCBI Accession #
AAB60503.1
NCBI Official Full Name
B7-1, partial
NCBI Official Synonym Full Names
Cd80 molecule
NCBI Official Symbol
Cd80
NCBI Official Synonym Symbols
B7-1
UniProt Protein Name
B7-1
UniProt Synonym Protein Names
B7-1
UniProt Gene Name
Cd80
UniProt Entry Name
Q62680_RAT
NCBI Protein Information
T-lymphocyte activation antigen CD80; CD80 antigen; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1)

NCBI Summary for B7-1

cell surface protein that is part of the immune response; may be involved in induction of experimental autoimmune anterior uveitis [RGD, Feb 2006]

Research Articles on B7-1

1. Selective expression of B7-1 (CD80) is important and may serve as a guide for future therapies aimed at transplant tolerance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-B7-1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
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