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ELF5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2402056

ELF5 antibody is an unconjugated rabbit polyclonal antibody to ELF5 (aa101-150) from mouse. It is reactive with mouse, rat, bat and other species. Validated for IHC and WB. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ELF5, Polyclonal Antibody
Full Product Name
ELF5 Rabbit anti-Mouse Polyclonal (aa101-150) Antibody
Product Gene Name
Product Synonym Names
IHC-plus ELF5 Antibody (aa101-150); ELF5; ESE2; E74-like factor 5; ESE-2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
PBS, 0.09% sodium azide, 2% sucrose
Purity/Purification
Immunoaffinity purified
Concentration
0.5mg/ml (lot specific)
Specificity
Mouse ELF5
Preparation and Storage
Short term: Store at 2-8 degree C. Long term: Aliquot and store at -20 degree C. Avoid freeze/thaw cycles.
Molecular Weight
18,866 Da
OMIM
605169
3D Structure
ModBase 3D Structure for Q9UKW6
Target
Mouse ELF5
Immunogen
Synthetic peptide located between aa101-150 of mouse Elf5 (Q8VDK3, NP_034255). Percent identity by BLAST analysis: Galago, Mouse, Rat, Elephant, Bat, Pig (100%); Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rabbit, Horse (92%); Orangutan, Tamarin (90%); Dog, Bovine (85%); Guinea pig (84%).
Conjugation
Unconjugated
Epitope
aa101-150
Applications Tested/Suitable for anti-ELF5 antibody
Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Application Notes for anti-ELF5 antibody
IHC-P: 5ug/ml
WB: 0.2-1ug/ml
Western Blot: Suggested dilution at 1ug/ml in 5% skim milk/PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000-100,000 as secondary antibody.

Western Blot (WB) of anti-ELF5 antibody

ELF5 Antibody-ELF5 antibody Western blot of Mouse Kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Rabbit Anti-ELF5 Polyclonal Antibody – Cat# MBS2402056

Immunohistochemistry (IHC) of anti-ELF5 antibody

ELF5 Antibody-Anti-ELF5 antibody IHC of mouse salivary glands. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Rabbit Anti-ELF5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2402056

Other Notes

Small volumes of anti-ELF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELF5 purchase

MBS2402056 is an antibody targeting ELF5. Also known as ELF5 Rabbit anti-Mouse Polyclonal (aa101-150) Antibody. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ELF5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ETS-related transcription factor Elf-5 isoform 2
NCBI Official Synonym Full Names
E74 like ETS transcription factor 5
NCBI Official Symbol
ELF5
NCBI Official Synonym Symbols
ESE2
UniProt Protein Name
ETS-related transcription factor Elf-5
UniProt Synonym Protein Names
E74-like factor 5; Epithelium-restricted ESE-1-related Ets factor; Epithelium-specific Ets transcription factor 2
UniProt Gene Name
ELF5
UniProt Synonym Gene Names
ESE2; ESE-2
UniProt Entry Name
ELF5_HUMAN
NCBI Protein Information
ETS-related transcription factor Elf-5

NCBI Summary for ELF5

The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
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UniProt Comments for ELF5

ELF5: Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Belongs to the ETS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p13-p12

Cellular Component: cytoplasm; nucleus

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell proliferation; ectoderm development; positive regulation of transcription from RNA polymerase II promoter; cell differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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