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GADD34/Ppp1r15a (PPP1R15A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755080

Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. Enables protein phosphatase binding activity and protein phosphatase regulator… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GADD34/Ppp1r15a, Polyclonal Antibody
Full Product Name
Anti-GADD34/Ppp1r15a Antibody Picoband
Product Gene Name
anti-PPP1R15A antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Molecular Weight
71,840 Da
Chromosome Location
Chromosome: 7; NC_000073.6 (45522917..45526268, complement). Location: 7 B4; 7
3D Structure
ModBase 3D Structure for P17564
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived mouse GADD34/Ppp1r15a recombinant protein (Position: R14-R572).

Product Categories/Family for anti-PPP1R15A antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PPP1R15A antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-PPP1R15A antibody
WB: 0.25-0.5 ug/ml, Mouse
FC/FACS: 1-3 ug/1x10^6 cells, Mouse
Direct ELISA: 0.1-0.5 ug/ml, Mouse
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Western Blot (WB) of anti-PPP1R15A antibody

Figure 1. Western blot analysis of GADD34/Ppp1r15a using anti-GADD34/Ppp1r15a antibody (MBS1755080).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: mouse stomach tissue lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/Ppp1r15a antigen affinity purified polyclonal antibody (#MBS1755080) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GADD34/Ppp1r15a at approximately 100 kDa. The expected band size for GADD34/Ppp1r15a is at 73 kDa.
Rabbit Anti-PPP1R15A Polyclonal Antibody – Cat# MBS1755080

Flow Cytometry (FC/FACS) of anti-PPP1R15A antibody

Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-GADD34/Ppp1r15a antibody (MBS1755080).

Overlay histogram showing ANA-1 cells stained with MBS1755080 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GADD34/Ppp1r15a Antibody (MBS1755080, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PPP1R15A Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1755080

Other Notes

Small volumes of anti-PPP1R15A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPP1R15A purchase

MBS1755080 is an antibody targeting PPP1R15A. Also known as Anti-GADD34/Ppp1r15a Antibody Picoband. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP1R15A. It may not necessarily be applicable to this product.

NCBI GI #
6678978
NCBI GeneID
17872
NCBI Accession #
NP_032680.1
UniProt Primary Accession #
P17564
UniProt Secondary Accession #
Q3TJS6; Q3U3L5; Q8C579
UniProt Related Accession #
P17564
GenBank Nucleotide #
NM_008654.2
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 15A
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory (inhibitor) subunit 15A
NCBI Official Symbol
Ppp1r15a
NCBI Official Synonym Symbols
Gadd34; Myd116; 9630030H21
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 15A
UniProt Synonym Protein Names
Growth arrest and DNA damage-inducible protein GADD34; Myeloid differentiation primary response protein MyD116
Protein Family
Protein phosphatase 1 regulatory
UniProt Gene Name
Ppp1r15a
UniProt Synonym Gene Names
Gadd34; Myd116
UniProt Entry Name
PR15A_MOUSE
NCBI Protein Information
protein phosphatase 1 regulatory subunit 15A; growth arrest and DNA-damage-inducible 34; myeloid differentiation primary response gene 116; growth arrest and DNA damage-inducible protein GADD34; myeloid differentiation primary response protein MyD116

UniProt Comments for PPP1R15A

GADD34: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Belongs to the PPP1R15 family.

Protein type: Apoptosis; Cell cycle regulation; DNA repair, damage; Translation initiation

Cellular Component: Golgi apparatus; mitochondrial outer membrane; mitochondrion; membrane; endoplasmic reticulum; cytoplasm; protein phosphatase type 1 complex

Molecular Function: protein phosphatase regulator activity; protein kinase binding; protein phosphatase binding; protein phosphatase 1 binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; regulation of translation; negative regulation of protein amino acid dephosphorylation; unfolded protein response; apoptosis; negative regulation of phosphoprotein phosphatase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase I promoter
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Research Articles on PPP1R15A

1. Data indicate that ATF4/GADD34 and p90ATF6 expression gradually changed, and the levels of cleaved caspase-3 were significantly increased with repetitive cycles of testicular hyperthermia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPP1R15A antibody

 Reference Product  PubMed Publications
 Atf4 antibody  >18 publications with PPP1R15A and Atf4
 Cdkn1a antibody  >8 publications with PPP1R15A and Cdkn1a
 Kif3a antibody  >1 publications with PPP1R15A and Kif3a
 Smad7 antibody  >1 publications with PPP1R15A and Smad7

Pathways associated with anti-PPP1R15A antibody

 Products by Pathway  Pathway Diagram
 Downregulation Of TGF-beta Receptor Signaling Pathway antibodies  Downregulation Of TGF-beta Receptor Signaling Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By TGF-beta Receptor Complex Pathway antibodies  Signaling By TGF-beta Receptor Complex Pathway Diagram
 TGF-beta Receptor Signaling Activates SMADs Pathway antibodies  TGF-beta Receptor Signaling Activates SMADs Pathway Diagram

Diseases associated with anti-PPP1R15A antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >49 publications with PPP1R15A and Neoplasms
 Necrosis Antibodies  >14 publications with PPP1R15A and Necrosis
 Disease Models, Animal Antibodies  >13 publications with PPP1R15A and Disease Models, Animal
 Nervous System Diseases Antibodies  >12 publications with PPP1R15A and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >8 publications with PPP1R15A and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >6 publications with PPP1R15A and Neoplasms, Experimental
 Liver Diseases Antibodies  >5 publications with PPP1R15A and Liver Diseases
 Inflammation Antibodies  >4 publications with PPP1R15A and Inflammation
 Lung Neoplasms Antibodies  >3 publications with PPP1R15A and Lung Neoplasms
 Fatty Liver Antibodies  >3 publications with PPP1R15A and Fatty Liver

Organs/Tissues associated with anti-PPP1R15A antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15 publications with PPP1R15A and Blood
 Brain Antibodies  >12 publications with PPP1R15A and Brain
 Liver Antibodies  >12 publications with PPP1R15A and Liver
 Lung Antibodies  >5 publications with PPP1R15A and Lung
 Skin Antibodies  >4 publications with PPP1R15A and Skin
 Embryonic Tissue Antibodies  >3 publications with PPP1R15A and Embryonic Tissue
 Lymph Node Antibodies  >2 publications with PPP1R15A and Lymph Node
 Intestine Antibodies  >2 publications with PPP1R15A and Intestine
 Testis Antibodies  >2 publications with PPP1R15A and Testis
 Thyroid Antibodies  >1 publications with PPP1R15A and Thyroid
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