Rabbit Anti-JUN Polyclonal Antibody – Cat# MBS647654

Dot blot analysis of Phospho-mouse JUN-T289 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

JUN Antibody – Rabbit Polyclonal

Name: JUN Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS647654
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647654

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

JUN, Polyclonal Antibody

Full Product Name

JUN, phosphorylated (T289) (Jun, Transcription factor AP-1, AH119, Activator protein 1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog)

Product Gene Name

anti-JUN antibody

Product Synonym Names

Anti -JUN, phosphorylated (T289) (Jun, Transcription factor AP-1, AH119, Activator protein 1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Mouse

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

35,676 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (59246463..59249785, complement). Location: 1p32-p31

OMIM

165160

3D Structure

ModBase 3D Structure for P05412

Immunogen

Phospho-mouse JUN-T289 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T289 of mouse JUN.

Product Categories/Family for anti-JUN antibody

Antibodies; Abs to Transcription Factors

Applications Tested/Suitable for anti-JUN antibody

ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)

Application Notes for anti-JUN antibody

Suitable for use in Dot Blot, ELISA
Dilution: ELISA: 1:1,000
Dot blot 1:500

Other Notes

Small volumes of anti-JUN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JUN purchase

MBS647654 is an antibody targeting JUN. Also known as JUN, phosphorylated (T289) (Jun, Transcription factor AP-1, AH119, Activator protein 1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog). Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for JUN. It may not necessarily be applicable to this product.

NCBI GI #

386839

NCBI GeneID

3725

NCBI Accession #

AAA59197.1

UniProt Primary Accession #

P05412

UniProt Secondary Accession #

Q96G93

UniProt Related Accession #

P05412

NCBI Official Full Name

JUN

NCBI Official Synonym Full Names

jun proto-oncogene

NCBI Official Symbol

JUN

NCBI Official Synonym Symbols

AP1; AP-1; c-Jun

UniProt Protein Name

Transcription factor AP-1

UniProt Synonym Protein Names

Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39

Protein Family

Transcription factor

UniProt Gene Name

JUN

UniProt Synonym Gene Names

AP1

UniProt Entry Name

JUN_HUMAN

NCBI Protein Information

transcription factor AP-1; p39; jun oncogene; activator protein 1; proto-oncogene c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; v-jun sarcoma virus 17 oncogene homolog; v-jun avian sarcoma virus 17 oncogene homolog

UniProt Comments for JUN

Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Ref.15 Ref.19

Subunit structure: Heterodimer with either FOS or BATF3 or ATF7. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA

By similarity. Interacts with HIVEP3 and MYBBP1A

By similarity. Interacts with SP1, SPIB and TCF20. Interacts with COPS5; the interaction leads indirectly to its phosphorylation. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex. Interacts with RNF187. Binds to HIPK3. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17 Ref.18 Ref.20 Ref.26

Subcellular location: Nucleus.

Post-translational modification: Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Ref.7 Ref.8 Ref.9 Ref.19 Ref.21 Ref.22 Ref.28 Ref.30Acetylated at Lys-271 by EP300.

Sequence similarities: Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP (basic-leucine zipper) domain.

Research Articles on JUN

1. Activator protein-1 has a role in multidrug resistance associated protein 3 upregulation by 17alpha-ethynylestradiol.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-JUN antibody

Reference Product	PubMed Publications
FOS antibody	>7799 publications with JUN and FOS
MAPK1 antibody	>1879 publications with JUN and MAPK1
UBC antibody	>821 publications with JUN and UBC
MAPK8 antibody	>767 publications with JUN and MAPK8
ATF2 antibody	>378 publications with JUN and ATF2
ATF3 antibody	>183 publications with JUN and ATF3
CREBBP antibody	>150 publications with JUN and CREBBP
EP300 antibody	>67 publications with JUN and EP300
FOSL1 antibody	>13 publications with JUN and FOSL1
MAPK9 antibody	>11 publications with JUN and MAPK9

Pathways associated with anti-JUN antibody

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies	Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
AhR Pathway antibodies	AhR Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram

Diseases associated with anti-JUN antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>215979 publications with JUN and Neoplasms
Nervous System Diseases Antibodies	>171978 publications with JUN and Nervous System Diseases
Cardiovascular Diseases Antibodies	>155196 publications with JUN and Cardiovascular Diseases
Brain Diseases Antibodies	>81108 publications with JUN and Brain Diseases
Heart Diseases Antibodies	>76519 publications with JUN and Heart Diseases
Pain Antibodies	>49445 publications with JUN and Pain
Liver Diseases Antibodies	>40893 publications with JUN and Liver Diseases
Inflammation Antibodies	>37897 publications with JUN and Inflammation
Kidney Diseases Antibodies	>35206 publications with JUN and Kidney Diseases
Hypertension Antibodies	>31980 publications with JUN and Hypertension

Organs/Tissues associated with anti-JUN antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>289684 publications with JUN and Blood
Brain Antibodies	>118938 publications with JUN and Brain
Heart Antibodies	>93340 publications with JUN and Heart
Vascular Antibodies	>84070 publications with JUN and Vascular
Bone Antibodies	>81350 publications with JUN and Bone
Liver Antibodies	>74569 publications with JUN and Liver
Muscle Antibodies	>72408 publications with JUN and Muscle
Lung Antibodies	>56205 publications with JUN and Lung
Kidney Antibodies	>54862 publications with JUN and Kidney
Skin Antibodies	>51894 publications with JUN and Skin