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MLKL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641814

MBS641814 is an antibody targeting MLKL. Also known as Mlkl, CT (Mlkl, Mixed lineage kinase domain-like protein). Reactive in Mouse. Host: Rabbit. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mlkl, Polyclonal Antibody
Full Product Name
Mlkl, CT (Mlkl, Mixed lineage kinase domain-like protein)
Product Gene Name
anti-MLKL antibody
Product Synonym Names
Anti -Mlkl, CT (Mlkl, Mixed lineage kinase domain-like protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse Mlkl.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
54,479 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (74705753..74734789, complement). Location: 16q23.1
3D Structure
ModBase 3D Structure for Q8NB16
Immunogen
Synthetic peptide corresponding to aa443-472 at the C-terminal of mouse Mlkl, conjugated to KLH. (NP_083281.1)

Product Categories/Family for anti-MLKL antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-MLKL antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-MLKL antibody
Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: 1:100-1:500

Western Blot (WB) of anti-MLKL antibody

Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS641814. This demonstrates that MBS641814 detected the Mlkl protein (arrow).
Rabbit Anti-MLKL Polyclonal Antibody – Cat# MBS641814

Testing Data of anti-MLKL antibody

All lanes: MBS641814 (1:2000) Lane 1: mouse testis lysates Lane 2: mouse liver lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000) Predicted band size: 54kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-MLKL Polyclonal Antibody, Testing Data – Cat# MBS641814

Testing Data of anti-MLKL antibody

All lanes: MBS641814 (1:2000) Lane 1: NIH/3T3 whole cell lysates Lane 2: mouse spleen lysates Lysates/proteins at 20ug/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 54kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-MLKL Polyclonal Antibody, Testing Data – Cat# MBS641814

Western Blot (WB) of anti-MLKL antibody

Western Blot analysis in mouse lung lysates (20ug) using MBS641814 (1:1000). Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 54kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-MLKL Polyclonal Antibody, Western Blot (WB) – Cat# MBS641814

Other Notes

Small volumes of anti-MLKL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MLKL. It may not necessarily be applicable to this product.

NCBI GI #
215820622
NCBI GeneID
197259
NCBI Accession #
NP_001135969.1
UniProt Primary Accession #
Q8NB16
UniProt Secondary Accession #
Q8N6V0; A6NCE4
UniProt Related Accession #
Q8NB16
GenBank Nucleotide #
NM_001142497.1
NCBI Official Full Name
mixed lineage kinase domain-like protein isoform 2
NCBI Official Synonym Full Names
mixed lineage kinase domain-like
NCBI Official Symbol
MLKL
UniProt Protein Name
Mixed lineage kinase domain-like protein
Protein Family
Mixed lineage kinase domain-like protein
UniProt Gene Name
MLKL
UniProt Entry Name
MLKL_HUMAN
NCBI Protein Information
mixed lineage kinase domain-like protein

NCBI Summary for MLKL

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for MLKL

MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; TKL-Unique family

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: protein amino acid phosphorylation
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Research Articles on MLKL

1. the importance of the RIP3-MLKL interaction in the formation of functional necrosomes and suggest that translocation of necrosomes to mitochondria-associated membranes is essential for necroptosis signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MLKL antibody

 Reference Product  PubMed Publications
 RIPK3 antibody  >9 publications with MLKL and RIPK3

Pathways associated with anti-MLKL antibody

 Products by Pathway  Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram

Diseases associated with anti-MLKL antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >15 publications with MLKL and Necrosis
 Inflammation Antibodies  >5 publications with MLKL and Inflammation
 Neoplasms Antibodies  >1 publications with MLKL and Neoplasms
 Immune System Diseases Antibodies  >1 publications with MLKL and Immune System Diseases
 Fatty Liver Antibodies  >1 publications with MLKL and Fatty Liver
 Drug-Induced Liver Injury Antibodies  >1 publications with MLKL and Drug-Induced Liver Injury

Organs/Tissues associated with anti-MLKL antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with MLKL and Muscle
 Uncharacterized Tissue Antibodies  >1 publications with MLKL and Uncharacterized Tissue
 Liver Antibodies  >1 publications with MLKL and Liver
 Blood Antibodies  >1 publications with MLKL and Blood
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