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NUDT9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6327183

MBS6327183 is an antibody targeting NUDT9. Also known as NUDT9, CT (NUDT9, NUDT10, ADP-ribose pyrophosphatase, mitochondrial, ADP-ribose diphosphatase, ADP-ribose phosphohydrolase, Adenosine diphosphoribose pyrophosphatase, Nucleoside diphosphate-linked… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NUDT9, Polyclonal Antibody
Full Product Name
NUDT9, CT (NUDT9, NUDT10, ADP-ribose pyrophosphatase, mitochondrial, ADP-ribose diphosphatase, ADP-ribose phosphohydrolase, Adenosine diphosphoribose pyrophosphatase, Nucleoside diphosphate-linked moiety X motif 9) (AP)
Product Gene Name
anti-NUDT9 antibody
Product Synonym Names
NUDT9; NUDT10; ADP-ribose pyrophosphatase, mitochondrial; ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; Adenosine diphosphoribose pyrophosphatase; Nucleoside diphosphate-linked moiety X motif 9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
606022
3D Structure
ModBase 3D Structure for Q9BW91
Immunogen
NUDT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-347 amino acids from the C-terminal region of human NUDT9.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-NUDT9 antibody

Antibodies; NUDT19
Applications Tested/Suitable for anti-NUDT9 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-NUDT9 antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NUDT9 antibody

NUDT9 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the NUDT9 antibody detected the NUDT9 protein (arrow).
Rabbit Anti-NUDT9 Polyclonal Antibody – Cat# MBS6327183

Immunohistochemistry (IHC) of anti-NUDT9 antibody

NUDT9 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUDT9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-NUDT9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6327183

Product References and Citations for anti-NUDT9 antibody

Chen, L.Y., et al. Taiwan J Obstet Gynecol 48(2):96-107(2009). Carloto, A., et al. Biochim. Biophys. Acta 1760(10):1545-1551(2006). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Shen, B.W., et al. J. Mol. Biol. 332(2):385-398(2003). Zhang, H.T., et al. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 35(8):747-751(2003).

Other Notes

Small volumes of anti-NUDT9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NUDT9. It may not necessarily be applicable to this product.

NCBI GI #
37594457
NCBI GeneID
53343
NCBI Accession #
NP_932155.1
UniProt Primary Accession #
Q9BW91
UniProt Related Accession #
Q9BW91
GenBank Nucleotide #
NP_932155.1
NCBI Official Full Name
ADP-ribose pyrophosphatase, mitochondrial isoform b
NCBI Official Synonym Full Names
nudix hydrolase 9
NCBI Official Symbol
NUDT9
NCBI Official Synonym Symbols
NUDT10
UniProt Protein Name
ADP-ribose pyrophosphatase, mitochondrial
UniProt Synonym Protein Names
ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase; Nucleoside diphosphate-linked moiety X motif 9; Nudix motif 9
Protein Family
Nudix hydrolase
UniProt Gene Name
NUDT9
UniProt Synonym Gene Names
NUDT10; ADPR-PPase; Nudix motif 9
UniProt Entry Name
NUDT9_HUMAN
NCBI Protein Information
ADP-ribose pyrophosphatase, mitochondrial

NCBI Summary for NUDT9

The protein encoded by this gene belongs to the Nudix hydrolase family. Nudix boxes are found in a family of diverse enzymes that catalyze the hydrolysis of nucleoside diphosphate derivatives. This enzyme is an ADP-ribose pyrophosphatase that catalyzes the hydrolysis of ADP-ribose to AMP and ribose-5-P. It requires divalent metal ions and an intact Nudix motif for enzymatic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for NUDT9

NUDT9: Hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5'- phosphate. Belongs to the Nudix hydrolase family. NudF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 3.6.1.13; Nucleotide Metabolism - purine; Hydrolase

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: mitochondrial matrix; intracellular

Molecular Function: ADP-ribose diphosphatase activity; ADP-sugar diphosphatase activity; adenosine-diphosphatase activity

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; ADP catabolic process; IDP catabolic process
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Research Articles on NUDT9

1. ADP-ribose reactive NUDT9 NUDIX hydrolase isoforms modulate HIF-1alpha in cancer cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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