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PIK3CA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8000770

MBS8000770 is an antibody targeting PIK3CA. Also known as PIK3CA Antibody: ATTO 390. Reactive in Mouse. Host: Rabbit. More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PIK3CA, Polyclonal Antibody
Full Product Name
PIK3CA Antibody: ATTO 390
Product Gene Name
anti-PIK3CA antibody
Product Synonym Names
Rabbit Anti-Human PIK3CA Polyclonal; PI 3 Kinase catalytic subunit alpha Antibody; Phosphoinositide 3 kinase catalytic alpha polypeptide Antibody; PI3 kinase p110 subunit alpha Antibody; PtdIns 3 kinase p110 Antibody; PtdIns 3 kinase regulatory subunit alpha Antibody; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 Antibody; GRB1 Antibody; PtdIns 3 kinase subunit p110 alpha Antibody; Phosphoinositide 3 kinase regulatory subunit 1 (alpha) Antibody; PtdIns-3-kinase subunit p110-alpha Antibody; PtdIns-3-kinase subunit alpha Antibody; PtdIns 3 kinase regulatory subunit p85 alpha Antibody; p110alpha Antibody; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform Antibody; Phosphatidylinositol 3 kinase regulatory 1 Antibody; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha Antibody; PIK3R1 Antibody; EC:2.7.11.1 Antibody; Phosphoinositide-3-kinase catalytic alpha polypeptide Antibody; PI3K alpha Antibody; PI3-kinase subunit alpha Antibody; Phosphatidylinositol 3 kinase catalytic alpha polypeptide Antibody; p110 alpha Antibody; Phosphatidylinositol 3 kinase regulatory subunit alpha Antibody; Phosphatidylinositol 3 kinase catalytic 110 KD alpha Antibody; 5-bisphosphate 3-kinase catalytic subunit alpha isoform Antibody; PI3K-alpha Antibody; PI3 kinase subunit alpha Antibody; MGC142163 Antibody; EC:2.7.1.153 Antibody; PI3 kinase regulatory subunit alpha Antibody; p85 alpha Antibody; PI3Kalpha Antibody; Phosphoinositide 3 kinase regulatory subunit 1 Antibody; MGC142161 Antibody; Serine/threonine protein kinase PIK3CA (EC:2.7.11.1) Antibody; 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha Antibody; PI3K Antibody; Phosphatidylinositol 3 kinase associated p 85 alpha Antibody; p85 Antibody; Phosphatidylinositol 3 kinase 85 kDa regulatory subunit alpha Antibody; Phosphatidylinositol 4; PI3K regulatory subunit alpha Antibody; PI3 kinase subunit p85 alpha Antibody; PIK3CA Antibody; PtdIns 3 kinase subunit alpha Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects 55.7 kDa.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: PIK3CA (MBS8000769)
ATTO 390 Conjugated Antibody: PIK3CA (MBS8000770)
Matching Pairs
Unconjugated Antibody: N/A (MBS8000770)
ATTO 390 Conjugated Antibody: PIK3CA (MBS8000770)
Molecular Weight
124,284 Da
OMIM
114480
3D Structure
ModBase 3D Structure for P42336
Target
PIK3CA
Immunogen
Synthetic peptide of Human PIK3CA (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Species
Human
Conjugate
ATTO 390
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide

Product Categories/Family for anti-PIK3CA antibody

Antibodies; Cancer; Tumor Biomarkers; Oncoproteins; Immunology; Innate Immunity; TLR Signaling
Applications Tested/Suitable for anti-PIK3CA antibody
Western Blot (WB)
Application Notes for anti-PIK3CA antibody
WB: 1:1000

Western Blot (WB) of anti-PIK3CA antibody

Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.
Rabbit Anti-PIK3CA Polyclonal Antibody – Cat# MBS8000770

Other Notes

Small volumes of anti-PIK3CA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PIK3CA. It may not necessarily be applicable to this product.

NCBI GI #
54792082
NCBI GeneID
5290
NCBI Accession #
NP_006209.2
UniProt Primary Accession #
P42336
UniProt Secondary Accession #
Q14CW1; Q99762
UniProt Related Accession #
P42336
GenBank Nucleotide #
NM_006218.3
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
NCBI Official Synonym Full Names
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
NCBI Official Symbol
PIK3CA
NCBI Official Synonym Symbols
MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
UniProt Synonym Protein Names
Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA (EC:2.7.11.1
Protein Family
Phosphatidylinositol 4,5-bisphosphate 3-kinase
UniProt Gene Name
PIK3CA
UniProt Synonym Gene Names
PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

NCBI Summary for PIK3CA

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]
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UniProt Comments for PIK3CA

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Plays a role in the positive regulation of phagocytosis and pinocytosis ().
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Research Articles on PIK3CA

1. PIK3CA gene mutations were not found; however, a synonymous single nucleotide polymorphism (SNP) [rs17849079] was observed frequently [35/96 (36.4%)] in oral cancer samples.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PIK3CA antibody

 Reference Product  PubMed Publications
 PTEN antibody  >893 publications with PIK3CA and PTEN
 NRAS antibody  >392 publications with PIK3CA and NRAS
 AKT1 antibody  >339 publications with PIK3CA and AKT1
 HRAS antibody  >165 publications with PIK3CA and HRAS
 PIK3R1 antibody  >82 publications with PIK3CA and PIK3R1
 RPS6KB1 antibody  >13 publications with PIK3CA and RPS6KB1
 IRS1 antibody  >13 publications with PIK3CA and IRS1
 IRS2 antibody  >10 publications with PIK3CA and IRS2
 RRAS antibody  >1 publications with PIK3CA and RRAS

Pathways associated with anti-PIK3CA antibody

 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AMP-activated Protein Kinase (AMPK) Signaling Pathway antibodies  AMP-activated Protein Kinase (AMPK) Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram

Diseases associated with anti-PIK3CA antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >667 publications with PIK3CA and Adenocarcinoma
 Colorectal Neoplasms Antibodies  >625 publications with PIK3CA and Colorectal Neoplasms
 Breast Neoplasms Antibodies  >618 publications with PIK3CA and Breast Neoplasms
 Lung Neoplasms Antibodies  >402 publications with PIK3CA and Lung Neoplasms
 Disease Progression Antibodies  >357 publications with PIK3CA and Disease Progression
 Neoplasm Metastasis Antibodies  >348 publications with PIK3CA and Neoplasm Metastasis
 Carcinoma, Squamous Cell Antibodies  >336 publications with PIK3CA and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >288 publications with PIK3CA and Neoplasms, Experimental
 Ovarian Neoplasms Antibodies  >212 publications with PIK3CA and Ovarian Neoplasms
 Sarcoma Antibodies  >140 publications with PIK3CA and Sarcoma

Organs/Tissues associated with anti-PIK3CA antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >515 publications with PIK3CA and Lung
 Blood Antibodies  >250 publications with PIK3CA and Blood
 Liver Antibodies  >154 publications with PIK3CA and Liver
 Lymph Node Antibodies  >147 publications with PIK3CA and Lymph Node
 Brain Antibodies  >140 publications with PIK3CA and Brain
 Vascular Antibodies  >122 publications with PIK3CA and Vascular
 Prostate Antibodies  >54 publications with PIK3CA and Prostate
 Ovary Antibodies  >41 publications with PIK3CA and Ovary
 Heart Antibodies  >37 publications with PIK3CA and Heart
 Kidney Antibodies  >28 publications with PIK3CA and Kidney
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