Rabbit Anti-PUMA Polyclonal Antibody – Cat# MBS6496137

Western Blot Analysis: Mouse adult brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with the Ab (1:250 dilution of this lot). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Puma (~21kD).

PUMA Antibody – Rabbit Polyclonal

Name: PUMA Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6496137
Price: 1335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6496137

MBS6496137 is an antibody targeting PUMA. Also known as PUMA, BH3 domain (p53 Upregulated Modulator of Apoptosis, BCL2 Binding Component 3, BCL-2 Binding Component 3, BBC3, JFY1, JFY-1, p53 Up-regulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1) (HRP).… More details

$1335 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PUMA, Polyclonal Antibody

Full Product Name

PUMA, BH3 domain (p53 Upregulated Modulator of Apoptosis, BCL2 Binding Component 3, BCL-2 Binding Component 3, BBC3, JFY1, JFY-1, p53 Up-regulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1) (HRP)

Product Gene Name

anti-PUMA antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

Purity/Purification

Purified by Protein G Affinity Chromatography.

Specificity

Recognizes the 21kD Puma BH3 domain protein. Species Crossreactivity: mouse.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

23 kDa

3D Structure

ModBase 3D Structure for Q99ML1

Immunogen

Synthetic peptide corresponding to the C-terminal region of human Puma. Cellular Localization: Mitochondrion. Species sequence homology: human.

Conjugate

HRP

Positive Control

Mouse brain lysate

Note

Preservative Free

Product Categories/Family for anti-PUMA antibody

Antibodies; Apoptosis

Applications Tested/Suitable for anti-PUMA antibody

Western Blot (WB)

Application Notes for anti-PUMA antibody

WB: 1:250
Applications are based on unconjugated antibody.

Other Notes

Small volumes of anti-PUMA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PUMA. It may not necessarily be applicable to this product.

NCBI GI #

15193488

NCBI GeneID

27113

NCBI Accession #

NP_055232

UniProt Primary Accession #

Q99ML1

UniProt Secondary Accession #

O00171; Q96PG9; B9EGI3

UniProt Related Accession #

Q96PG8; Q9BXH1

GenBank Nucleotide #

NM_014417.4

NCBI Official Full Name

bcl-2-binding component 3 isoform 4

NCBI Official Synonym Full Names

BCL2 binding component 3

NCBI Official Symbol

BBC3

NCBI Official Synonym Symbols

JFY1; PUMA; JFY-1

UniProt Protein Name

Bcl-2-binding component 3

UniProt Synonym Protein Names

p53 up-regulated modulator of apoptosis

Protein Family

Hydroxycarboxylic acid receptor

UniProt Gene Name

Bbc3

UniProt Synonym Gene Names

Puma

UniProt Entry Name

BBC3_MOUSE

NCBI Protein Information

bcl-2-binding component 3; p53 up-regulated modulator of apoptosis

NCBI Summary for PUMA

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

UniProt Comments for PUMA

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: mitochondrial envelope; mitochondrion; endoplasmic reticulum; intracellular; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; positive regulation of protein homooligomerization; determination of adult life span; release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; negative regulation of cell growth; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of growth

Research Articles on PUMA

1. c-Jun transactivates Puma gene expression to promote osteoarthritis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PUMA antibody

Products by Pathway	Pathway Diagram
Activation Of BH3-only Proteins Pathway antibodies	Activation Of BH3-only Proteins Pathway Diagram
Activation Of PUMA And Translocation To Mitochondria Pathway antibodies	Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies	BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
DNA Damage Response Pathway antibodies	DNA Damage Response Pathway Diagram
DNA Damage Response (only ATM Dependent) Pathway antibodies	DNA Damage Response (only ATM Dependent) Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram

Diseases associated with anti-PUMA antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>73 publications with PUMA and Carcinoma
Adenocarcinoma Antibodies	>33 publications with PUMA and Adenocarcinoma
Breast Neoplasms Antibodies	>32 publications with PUMA and Breast Neoplasms
Lung Neoplasms Antibodies	>24 publications with PUMA and Lung Neoplasms
Necrosis Antibodies	>20 publications with PUMA and Necrosis
Liver Diseases Antibodies	>18 publications with PUMA and Liver Diseases
Lung Diseases Antibodies	>17 publications with PUMA and Lung Diseases
Nervous System Diseases Antibodies	>16 publications with PUMA and Nervous System Diseases
Carcinoma, Squamous Cell Antibodies	>13 publications with PUMA and Carcinoma, Squamous Cell
Brain Diseases Antibodies	>13 publications with PUMA and Brain Diseases

Organs/Tissues associated with anti-PUMA antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>30 publications with PUMA and Lung
Brain Antibodies	>18 publications with PUMA and Brain
Kidney Antibodies	>13 publications with PUMA and Kidney
Skin Antibodies	>12 publications with PUMA and Skin
Prostate Antibodies	>12 publications with PUMA and Prostate
Stomach Antibodies	>12 publications with PUMA and Stomach
Muscle Antibodies	>9 publications with PUMA and Muscle
Bone Antibodies	>9 publications with PUMA and Bone
Eye Antibodies	>7 publications with PUMA and Eye
Intestine Antibodies	>6 publications with PUMA and Intestine