Caspase 3 (CASP3) Antibody – Rabbit Polyclonal

This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 8 B1.1|8 26.39 cM

Cellular Component: cytoplasm; cytosol; membrane raft; nucleus

Molecular Function: aspartic-type endopeptidase activity; cyclin-dependent protein serine/threonine kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; hydrolase activity; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; cell fate commitment; cellular response to DNA damage stimulus; cellular response to organic substance; erythrocyte differentiation; heart development; intrinsic apoptotic signaling pathway in response to osmotic stress; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cyclin-dependent protein serine/threonine kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of neuron apoptotic process; protein processing; proteolysis; response to glucose stimulus; response to hydrogen peroxide; response to organic substance; response to UV; response to wounding; sensory perception of sound; T cell homeostasis