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EME1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9128701
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This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EME1, Polyclonal Antibody
Full Product Name
EME1 Polyclonal Antibody
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
63kDa
OMIM
610885
3D Structure
ModBase 3D Structure for Q96AY2
Immunogen
Recombinant protein of human EME1
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-EME1 antibody
Western Blot (WB)
Application Notes for anti-EME1 antibody
WB 1:500 - 1:2000

Western Blot (WB) of anti-EME1 antibody

Western blot analysis of extracts of various cell lines, using EME1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-EME1 Polyclonal Antibody – Cat# MBS9128701

Other Notes

Small volumes of anti-EME1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EME1 purchase

MBS9128701 is an antibody targeting EME1. Also known as EME1 Polyclonal Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EME1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
crossover junction endonuclease EME1 isoform 1
NCBI Official Synonym Full Names
essential meiotic structure-specific endonuclease 1
NCBI Official Symbol
EME1
NCBI Official Synonym Symbols
MMS4L; SLX2A
UniProt Protein Name
Crossover junction endonuclease EME1
UniProt Synonym Protein Names
MMS4 homolog; hMMS4
UniProt Gene Name
EME1
UniProt Synonym Gene Names
MMS4; hMMS4
UniProt Entry Name
EME1_HUMAN
NCBI Protein Information
crossover junction endonuclease EME1

UniProt Comments for EME1

EME1: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. Belongs to the EME1/MMS4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 3.1.22.-; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: cytoplasm; nucleolus; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; endonuclease activity

Biological Process: DNA repair; DNA recombination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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