Rabbit Anti-HRK Polyclonal Antibody – Cat# MBS221467

Western blot analysis of mouse pancreas lysate probed with Rabbit anti Human Hrk (MBS221467) at 2.5(A) and 5(B) ug/ml

HRK Antibody – Rabbit Polyclonal

Name: HRK Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS221467
Price: 615 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS221467

MBS221467 detects Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction… More details

$615 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HRK, Polyclonal Antibody

Full Product Name

RABBIT ANTI HRK

Product Gene Name

anti-HRK antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse, Rat

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 1.0mg/ml (lot specific)

Specificity

This item detects Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation.
Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight, HRK often migrates at 12-15kD.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

9,884 Da

OMIM

603447

3D Structure

ModBase 3D Structure for O00198

Perservative Stabilisers

0.02% Sodium Azide

Immunogen

15 amino acid peptide from near the centre of human HRK (NP_003797).
Buffer Solution||Phosphate buffered saline

Antiserum Preparation

Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-HRK antibody

Western Blot (WB)

Application Notes for anti-HRK antibody

Western Blot: This item detects a band of approximately 13kDa in mouse pancreas tissue lysate.
Western Blotting: Minimum Dilution: 2; Maximum Dilution: 5ug/ml

Other Notes

Small volumes of anti-HRK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HRK purchase

MBS221467 is an antibody targeting HRK. Also known as RABBIT ANTI HRK. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HRK. It may not necessarily be applicable to this product.

NCBI GI #

4504493

NCBI GeneID

8739

NCBI Accession #

NP_003797.1

UniProt Primary Accession #

O00198

UniProt Related Accession #

O00198

GenBank Nucleotide #

NM_003806.2

NCBI Official Full Name

activator of apoptosis harakiri

NCBI Official Synonym Full Names

harakiri, BCL2 interacting protein

NCBI Official Symbol

HRK

NCBI Official Synonym Symbols

DP5; HARAKIRI

UniProt Protein Name

Activator of apoptosis harakiri

UniProt Synonym Protein Names

BH3-interacting domain-containing protein 3; Neuronal death protein DP5

Protein Family

Activator of apoptosis harakiri

UniProt Gene Name

HRK

UniProt Synonym Gene Names

BID3

UniProt Entry Name

HRK_HUMAN

NCBI Protein Information

activator of apoptosis harakiri; BCL2-interacting protein; BH3-interacting domain-containing protein 3; activator of apoptosis Hrk; death protein 5; harakiri, BCL2 interacting protein (contains only BH3 domain); neuronal death protein DP5

NCBI Summary for HRK

This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

UniProt Comments for HRK

HRK: Promotes apoptosis.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: mitochondrion; integral to membrane

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; positive regulation of protein homooligomerization

Research Articles on HRK

1. our findings suggest that induction of the BH3-only protein Hrk is a critical step in 2-ME activation of the JNK-induced apoptotic pathway, targeting mitochondria by liberating proapoptotic protein Bak.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HRK antibody

Reference Product	PubMed Publications
JUN antibody	>32 publications with HRK and JUN
BAD antibody	>17 publications with HRK and BAD
BCL2L1 antibody	>14 publications with HRK and BCL2L1
BIK antibody	>10 publications with HRK and BIK
BMF antibody	>8 publications with HRK and BMF

Pathways associated with anti-HRK antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram

Diseases associated with anti-HRK antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>20 publications with HRK and Nervous System Diseases
Brain Diseases Antibodies	>8 publications with HRK and Brain Diseases
Inflammation Antibodies	>6 publications with HRK and Inflammation
Cardiovascular Diseases Antibodies	>5 publications with HRK and Cardiovascular Diseases
Breast Neoplasms Antibodies	>5 publications with HRK and Breast Neoplasms
Necrosis Antibodies	>5 publications with HRK and Necrosis
Disease Models, Animal Antibodies	>3 publications with HRK and Disease Models, Animal
Mitochondrial Diseases Antibodies	>3 publications with HRK and Mitochondrial Diseases
Seizures Antibodies	>2 publications with HRK and Seizures
Hepatitis Antibodies	>2 publications with HRK and Hepatitis

Organs/Tissues associated with anti-HRK antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>22 publications with HRK and Brain
Lymph Node Antibodies	>2 publications with HRK and Lymph Node
Testis Antibodies	>1 publications with HRK and Testis
Thyroid Antibodies	>1 publications with HRK and Thyroid