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ID1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1754846

DNA-binding protein inhibitor ID-1 is a protein that in humans is encoded by the ID1 gene. The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Id1, Polyclonal Antibody
Full Product Name
Anti-Id1 Antibody Picoband
Product Gene Name
anti-ID1 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Molecular Weight
15,560 Da
3D Structure
ModBase 3D Structure for P20067
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived mouse Id1 recombinant protein (Position: C16-I168).

Product Categories/Family for anti-ID1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ID1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ID1 antibody
WB: 0.1-0.25 ug/ml, Mouse, Rat
Direct ELISA: 0.1-0.5 ug/ml, Mouse
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Western Blot (WB) of anti-ID1 antibody

Figure 1. Western blot analysis of ID1 using anti-ID1 antibody (MBS1754846).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: rat pancreas tissue lysates,
Lane 2: mouse pancreas tissue lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ID1 antigen affinity purified polyclonal antibody (#MBS1754846) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ID1 at approximately 25 kDa. The expected band size for ID1 is at 25 kDa.
Rabbit Anti-ID1 Polyclonal Antibody – Cat# MBS1754846

Other Notes

Small volumes of anti-ID1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ID1 purchase

MBS1754846 is an antibody targeting ID1. Also known as Anti-Id1 Antibody Picoband. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ID1. It may not necessarily be applicable to this product.

NCBI GI #
2827752
NCBI GeneID
15901
NCBI Accession #
P20067.3
UniProt Primary Accession #
P20067
UniProt Secondary Accession #
Q61101; Q9D897
UniProt Related Accession #
P20067
NCBI Official Full Name
DNA-binding protein inhibitor ID-1
NCBI Official Synonym Full Names
inhibitor of DNA binding 1
NCBI Official Symbol
Id1
NCBI Official Synonym Symbols
Idb1; bHLHb24; AI323524; D2Wsu140e
UniProt Protein Name
DNA-binding protein inhibitor ID-1
UniProt Synonym Protein Names
Inhibitor of DNA binding 1; Inhibitor of differentiation 1
Protein Family
DNA-binding protein inhibitor
UniProt Gene Name
Id1
UniProt Synonym Gene Names
Id; Id-1; Idb1
NCBI Protein Information
DNA-binding protein inhibitor ID-1

UniProt Comments for ID1

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
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Research Articles on ID1

1. a loss of CULLIN3 represents a common signaling node for controlling the activity of intracellular WNT and SHH signaling pathways mediated by ID1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ID1 antibody

 Reference Product  PubMed Publications
 Myod1 antibody  >8 publications with ID1 and Myod1
 Tcf12 antibody  >6 publications with ID1 and Tcf12

Pathways associated with anti-ID1 antibody

 Products by Pathway  Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Oncogene Induced Senescence Pathway antibodies  Oncogene Induced Senescence Pathway Diagram
 PluriNetWork Pathway antibodies  PluriNetWork Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram

Organs/Tissues associated with anti-ID1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >98 publications with ID1 and Muscle
 Lung Antibodies  >75 publications with ID1 and Lung
 Brain Antibodies  >74 publications with ID1 and Brain
 Liver Antibodies  >64 publications with ID1 and Liver
 Prostate Antibodies  >63 publications with ID1 and Prostate
 Embryonic Tissue Antibodies  >60 publications with ID1 and Embryonic Tissue
 Bone Marrow Antibodies  >49 publications with ID1 and Bone Marrow
 Heart Antibodies  >36 publications with ID1 and Heart
 Skin Antibodies  >35 publications with ID1 and Skin
 Kidney Antibodies  >34 publications with ID1 and Kidney
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