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IGFBP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751340

Description: Rabbit IgG polyclonal antibody for IGFBP2 detection. Tested with WB, IHC-P, Direct ELISA in Mouse; Rat.Background: The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IGFBP2, Polyclonal Antibody
Full Product Name
Anti-IGFBP2 Antibody
Product Gene Name
anti-IGFBP2 antibody
Product Synonym Names
Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; BRL-BP; Igfbp2; Igfbp-2; Insulin-like growth factor binding protein 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
32,855 Da
3D Structure
ModBase 3D Structure for P12843
Immunogen
E Coli-derived rat IGFBP2 recombinant protein (Position: E35-Q304).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IGFBP2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-IGFBP2 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-IGFBP2 antibody

Figure 1. Western blot analysis of IGFBP2 using anti-IGFBP2 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse Neuro-2a whole cell lysates,
Lane 3: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 40KD. The expected band size for IGFBP2 is at 35KD.
Rabbit Anti-IGFBP2 Polyclonal Antibody – Cat# MBS1751340

Immunohistochemistry (IHC) of anti-IGFBP2 antibody

Figure 3. IHC analysis of IGFBP2 using anti-IGFBP2 antibody.
IGFBP2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-IGFBP2 antibody.
IGFBP2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-IGFBP2 antibody.
IGFBP2 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IGFBP2 antibody.
IGFBP2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IGFBP2 antibody.
IGFBP2 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IGFBP2 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-IGFBP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751340

Product References and Citations for anti-IGFBP2 antibody

1. Chesik D, De Keyser J, Wilczak N (2007). "Insulin-like growth factor binding protein-2 as a regulator of IGF actions in CNS: implications in multiple sclerosis.". Cytokine Growth Factor Rev. 18 (3-4): 267-78. 2. Wolf E, Lahm H, Wu M et al. (2000). "Effects of IGFBP-2 overexpression in vitro and in vivo.".Pediatr. Nephrol. 14 (7): 572-8. 3. Zapf J, Kiefer M, Merryweather J, Musiarz F, Bauer D, Born W, Fischer JA, Froesch ER (Oct 1990). "Isolation from adult human serum of four insulin-like growth factor (IGF) binding proteins and molecular cloning of one of them that is increased by IGF I administration and in extrapancreatic tumor hypoglycemia".J Biol Chem 265 (25): 14892-8.

Other Notes

Small volumes of anti-IGFBP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGFBP2 purchase

MBS1751340 is an antibody targeting IGFBP2. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IGFBP2. It may not necessarily be applicable to this product.

NCBI GI #
148747421
NCBI GeneID
25662
NCBI Accession #
NP_037254.2
UniProt Primary Accession #
P12843
UniProt Secondary Accession #
Q569C7
UniProt Related Accession #
P12843
GenBank Nucleotide #
NM_013122.2
NCBI Official Full Name
insulin-like growth factor-binding protein 2
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 2
NCBI Official Symbol
Igfbp2
NCBI Official Synonym Symbols
IBP-2; BRL-BP; IGFBP-2; ILGFBPA
UniProt Protein Name
Insulin-like growth factor-binding protein 2
UniProt Synonym Protein Names
BRL-BP
Protein Family
Insulin-like growth factor-binding protein
UniProt Gene Name
Igfbp2
UniProt Synonym Gene Names
Igfbp-2; IBP-2; IGF-binding protein 2; IGFBP-2
NCBI Protein Information
insulin-like growth factor-binding protein 2

NCBI Summary for IGFBP2

protein that binds to Igf-I and Igf-II [RGD, Feb 2006]

UniProt Comments for IGFBP2

Inhibits IGF-mediated growth and developmental rates (). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Research Articles on IGFBP2

1. Insulin-like growth factor 2 (IGF2) is an important regulator of fetal growth.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IGFBP2 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram

Diseases associated with anti-IGFBP2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >352 publications with IGFBP2 and Neoplasms
 Nervous System Diseases Antibodies  >95 publications with IGFBP2 and Nervous System Diseases
 Brain Diseases Antibodies  >80 publications with IGFBP2 and Brain Diseases
 Insulin Resistance Antibodies  >63 publications with IGFBP2 and Insulin Resistance
 Adenocarcinoma Antibodies  >49 publications with IGFBP2 and Adenocarcinoma
 Obesity Antibodies  >49 publications with IGFBP2 and Obesity
 Kidney Diseases Antibodies  >49 publications with IGFBP2 and Kidney Diseases
 Breast Neoplasms Antibodies  >47 publications with IGFBP2 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >45 publications with IGFBP2 and Cardiovascular Diseases
 Liver Diseases Antibodies  >38 publications with IGFBP2 and Liver Diseases

Organs/Tissues associated with anti-IGFBP2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >182 publications with IGFBP2 and Liver
 Brain Antibodies  >148 publications with IGFBP2 and Brain
 Ovary Antibodies  >122 publications with IGFBP2 and Ovary
 Prostate Antibodies  >76 publications with IGFBP2 and Prostate
 Embryonic Tissue Antibodies  >64 publications with IGFBP2 and Embryonic Tissue
 Heart Antibodies  >39 publications with IGFBP2 and Heart
 Placenta Antibodies  >37 publications with IGFBP2 and Placenta
 Eye Antibodies  >25 publications with IGFBP2 and Eye
 Adipose Tissue Antibodies  >21 publications with IGFBP2 and Adipose Tissue
 Pituitary Gland Antibodies  >18 publications with IGFBP2 and Pituitary Gland
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