Rabbit Anti-IGFBP3 Polyclonal Antibody – Cat# MBS1750405

Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (MBS1750405).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 40KD. The expected band size for IGFBP3 is at 32KD.

IGFBP3 Antibody – Rabbit Polyclonal

Name: IGFBP3 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750405
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750405

Description: IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IGFBP3, Polyclonal Antibody

Full Product Name

Anti-IGFBP3 Picoband Antibody

Product Gene Name

anti-IGFBP3 antibody

Product Synonym Names

Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3; Igfbp3; Igfbp-3

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Purity/Purification

Immunogen affinity purified

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

31,680 Da

3D Structure

ModBase 3D Structure for P15473

Immunogen

E Coli-derived rat IGFBP3 recombinant protein (Position: A29-K268).

Subcellular Localization

Secreted.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-IGFBP3 antibody

Cell Biology; Apoptosis; Receptors; Associated Proteins; Signal Transduction; Protein Phosphorylation; tyrosine Phosphatases; Cardiovascular; Angiogenesis; Angiogenic Factors; Growth Factors/Hormones; insulin/insulin-like; Atherosclerosis; Diabetes Associ

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-IGFBP3 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-IGFBP3 antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-IGFBP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGFBP3 purchase

MBS1750405 is an antibody targeting IGFBP3. Also known as Anti-IGFBP3 Picoband Antibody. Reactive in Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IGFBP3. It may not necessarily be applicable to this product.

NCBI GI #

298231182

NCBI GeneID

24484

NCBI Accession #

NP_036720.2

UniProt Primary Accession #

P15473

UniProt Related Accession #

P15473

GenBank Nucleotide #

NM_012588.2

NCBI Official Full Name

insulin-like growth factor-binding protein 3

NCBI Official Synonym Full Names

insulin-like growth factor binding protein 3

NCBI Official Symbol

Igfbp3

NCBI Official Synonym Symbols

IGF-BP3

UniProt Protein Name

Insulin-like growth factor-binding protein 3

Protein Family

Insulin-like growth factor-binding protein

UniProt Gene Name

Igfbp3

UniProt Synonym Gene Names

Igfbp-3; IBP-3; IGF-binding protein 3; IGFBP-3

NCBI Protein Information

insulin-like growth factor-binding protein 3

NCBI Summary for IGFBP3

an insulin growth factor (Igf) binding protein; involved in modulating IGF-I action [RGD, Feb 2006]

UniProt Comments for IGFBP3

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Research Articles on IGFBP3

1. Compound 49b treatment inhibited these retinal changes. In REC cultured in hypoxia, levels of IGFBP-3 were reduced, which were significantly increased by Compound 49b.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IGFBP3 antibody

Reference Product	PubMed Publications
Igf1 antibody	>251 publications with IGFBP3 and Igf1
Igf2 antibody	>83 publications with IGFBP3 and Igf2
Igfals antibody	>21 publications with IGFBP3 and Igfals
Tp53 antibody	>13 publications with IGFBP3 and Tp53
Ins2 antibody	>2 publications with IGFBP3 and Ins2
Mapk9 antibody	>1 publications with IGFBP3 and Mapk9
Mapk8 antibody	>1 publications with IGFBP3 and Mapk8

Pathways associated with anti-IGFBP3 antibody

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Myometrial Relaxation And Contraction Pathways antibodies	Myometrial Relaxation And Contraction Pathways Diagram
Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies	Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
P53 Pathway antibodies	P53 Pathway Diagram
P53 Signal Pathway antibodies	P53 Signal Pathway Diagram
P53 Signaling Pathway antibodies	P53 Signaling Pathway Diagram
P53 Signaling Pathway antibodies	P53 Signaling Pathway Diagram

Diseases associated with anti-IGFBP3 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>363 publications with IGFBP3 and Breast Neoplasms
Prostatic Neoplasms Antibodies	>267 publications with IGFBP3 and Prostatic Neoplasms
Cardiovascular Diseases Antibodies	>246 publications with IGFBP3 and Cardiovascular Diseases
Liver Diseases Antibodies	>156 publications with IGFBP3 and Liver Diseases
Colorectal Neoplasms Antibodies	>155 publications with IGFBP3 and Colorectal Neoplasms
Lung Diseases Antibodies	>148 publications with IGFBP3 and Lung Diseases
Weight Gain Antibodies	>148 publications with IGFBP3 and Weight Gain
Inflammation Antibodies	>141 publications with IGFBP3 and Inflammation
Kidney Diseases Antibodies	>125 publications with IGFBP3 and Kidney Diseases
Disease Models, Animal Antibodies	>123 publications with IGFBP3 and Disease Models, Animal

Organs/Tissues associated with anti-IGFBP3 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>447 publications with IGFBP3 and Liver
Muscle Antibodies	>308 publications with IGFBP3 and Muscle
Connective Tissue Antibodies	>226 publications with IGFBP3 and Connective Tissue
Brain Antibodies	>217 publications with IGFBP3 and Brain
Kidney Antibodies	>209 publications with IGFBP3 and Kidney
Lung Antibodies	>194 publications with IGFBP3 and Lung
Ovary Antibodies	>160 publications with IGFBP3 and Ovary
Heart Antibodies	>151 publications with IGFBP3 and Heart
Placenta Antibodies	>82 publications with IGFBP3 and Placenta
Embryonic Tissue Antibodies	>80 publications with IGFBP3 and Embryonic Tissue