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Merlin (NF2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9601852

Description: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Merlin (NF2), Polyclonal Antibody
Full Product Name
Merlin Antibody
Product Gene Name
anti-NF2 antibody
Product Synonym Names
ACN; BANF; Bilateral acoustic neuroma; MERL_HUMAN; Merlin; Moesin ezrin radixin like protein; Moesin ezrin radizin like; Moesin-ezrin-radixin-like protein; Neurofibromatosis 2; Neurofibromatosis type 2; Neurofibromatosis2; Neurofibromin 2; Neurofibromin-2; Neurofibromin2; NF 2; Nf2; SCH; Schwannomerlin; Schwannomin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
Merlin antibody detects endogenous levels of total Merlin
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
NF2 / Merlin peptide (MBS9617402) is used for blocking the activity of NF2 / Merlin antibody (MBS9601852)
Molecular Weight
Observed: 70 kDa
Predicted: 70 kDa
3D Structure
ModBase 3D Structure for P35240
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Merlin
Subcellular Location
Cytoplasm > Perinuclear Region. Cytoplasmic Granule. Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia; Cytoplasm > Perinuclear Region. Cytoplasmic Granule. Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia; Nucleus. Cell Projection > Filopodium Membrane. Cell Projection > Ruffle Membrane. Cytoplasm > Perinuclear Region. Cytoplasmic Granule. Cytoplasm > Cytoskeleton. In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia and Cell Projection > Filopodium Membrane. Cell Projection > Ruffle Membrane. Nucleus. In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.
Tissue Specificity
Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NF2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-NF2 antibody
WB: 1:500-1:2000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-NF2 antibody

MBS9601852 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-NF2 Polyclonal Antibody – Cat# MBS9601852

Western Blot (WB) of anti-NF2 antibody

Western blot analysis of Merlin expression in IFN-alpha treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-NF2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9601852

Other Notes

Small volumes of anti-NF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NF2 purchase

MBS9601852 is an antibody targeting NF2. Also known as Merlin Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NF2. It may not necessarily be applicable to this product.

NCBI GI #
4557795
NCBI GeneID
4771
NCBI Accession #
NP_000259.1
UniProt Primary Accession #
P35240
UniProt Secondary Accession #
O95683; Q8WUJ2; Q969N0; Q969Q3; Q96T30; Q96T31; Q96T32; Q96T33; Q9BTW3; Q9UNG9; Q9UNH3
UniProt Related Accession #
P35240
GenBank Nucleotide #
NM_000268.3
NCBI Official Full Name
merlin isoform 1
NCBI Official Synonym Full Names
neurofibromin 2
NCBI Official Symbol
NF2
NCBI Official Synonym Symbols
ACN; SCH; BANF
UniProt Protein Name
Merlin
UniProt Synonym Protein Names
Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin
Protein Family
Merlin
UniProt Gene Name
NF2
UniProt Synonym Gene Names
SCH
NCBI Protein Information
merlin

NCBI Summary for NF2

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]
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UniProt Comments for NF2

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
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Research Articles on NF2

1. Merlin loss increased oxidative stress causing aberrant activation of Hedgehog signaling in in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NF2 antibody

 Reference Product  PubMed Publications
 ERBB2 antibody  >17 publications with NF2 and ERBB2
 CD44 antibody  >16 publications with NF2 and CD44
 PAK1 antibody  >9 publications with NF2 and PAK1
 CTNNB1 antibody  >8 publications with NF2 and CTNNB1
 TSC1 antibody  >4 publications with NF2 and TSC1
 SCHIP1 antibody  >3 publications with NF2 and SCHIP1
 RALGDS antibody  >2 publications with NF2 and RALGDS
 PXN antibody  >2 publications with NF2 and PXN
 SDCBP antibody  >1 publications with NF2 and SDCBP
 SPTBN1 antibody  >1 publications with NF2 and SPTBN1

Pathways associated with anti-NF2 antibody

 Products by Pathway  Pathway Diagram
 ErbB2/ErbB3 Signaling Events Pathway antibodies  ErbB2/ErbB3 Signaling Events Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with anti-NF2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >984 publications with NF2 and Neoplasms
 Nervous System Diseases Antibodies  >866 publications with NF2 and Nervous System Diseases
 Neurofibromatosis 2 Antibodies  >776 publications with NF2 and Neurofibromatosis 2
 Meningioma Antibodies  >246 publications with NF2 and Meningioma
 Schwannomatosis Antibodies  >74 publications with NF2 and Schwannomatosis
 Mesothelioma Antibodies  >57 publications with NF2 and Mesothelioma
 Meningioma, familial Antibodies  >54 publications with NF2 and Meningioma, familial
 Eye Diseases Antibodies  >50 publications with NF2 and Eye Diseases
 Skin Neoplasms Antibodies  >43 publications with NF2 and Skin Neoplasms
 Carcinoma Antibodies  >38 publications with NF2 and Carcinoma

Organs/Tissues associated with anti-NF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >276 publications with NF2 and Brain
 Skin Antibodies  >60 publications with NF2 and Skin
 Eye Antibodies  >45 publications with NF2 and Eye
 Bone Antibodies  >34 publications with NF2 and Bone
 Lung Antibodies  >30 publications with NF2 and Lung
 Muscle Antibodies  >29 publications with NF2 and Muscle
 Vascular Antibodies  >26 publications with NF2 and Vascular
 Liver Antibodies  >15 publications with NF2 and Liver
 Kidney Antibodies  >15 publications with NF2 and Kidney
 Embryonic Tissue Antibodies  >11 publications with NF2 and Embryonic Tissue
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