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Osteocalcin (OCN) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9603531

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Osteocalcin (OCN), Polyclonal Antibody
Full Product Name
Osteocalcin Antibody
Product Gene Name
Product Synonym Gene Name
BGLAP
Product Synonym Names
BGLAP; BGP; Bone gamma carboxyglutamate (gla) protein; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Gamma carboxyglutamic acid containing protein; Gamma-carboxyglutamic acid-containing protein; OC; OSTCN_HUMAN; Osteocalcin; OTTHUMP00000016586; PMF1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Peptide affinity purification
Concentration
1mg/ml (lot specific)
Specificity
Osteocalcin antibody detects endogenous levels of Osteocalcin.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Molecular Weight
11 kDa
3D Structure
ModBase 3D Structure for P02818
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Osteocalcin, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-OCN antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-OCN antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-500
*The optimal dilutions should be determined by the end user.

IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.

Western Blot (WB) of anti-OCN antibody

Western blot analysis of extracts from rat brain, using Osteocalcin antibody. Lane 1 was treated with the blocking peptide.
Rabbit Anti-OCN Polyclonal Antibody – Cat# MBS9603531

Immunohistochemistry (IHC) of anti-OCN antibody

MBS9603531 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated goat anti-rabbit antibody was used as the secondary Ab.
Rabbit Anti-OCN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9603531

Other Notes

Small volumes of anti-OCN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OCN purchase

MBS9603531 is an antibody targeting OCN. Also known as Osteocalcin Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OCN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma-carboxyglutamate protein
NCBI Official Symbol
BGLAP
NCBI Official Synonym Symbols
OC; BGP; OCN
UniProt Protein Name
Osteocalcin
UniProt Synonym Protein Names
Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
Protein Family
UniProt Gene Name
BGLAP
UniProt Synonym Gene Names
BGP
NCBI Protein Information
osteocalcin

NCBI Summary for OCN

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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