• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Osteoblast Specific Factor 2 (OSF-2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6491185

MBS6491185 is an antibody targeting OSF-2. Also known as Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like) (MaxLight 550). Reactive in Human, Rat. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Osteoblast Specific Factor 2 (OSF-2), Polyclonal Antibody
Full Product Name
Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like) (MaxLight 550)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human Osteoblast Specific Factor 2. Species Crossreactivity: rat
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
80,357 Da
OMIM
608777
3D Structure
ModBase 3D Structure for Q15063
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 687-716 in the C-terminal region of human Osteoblast Specific Factor 2.
Conjugate
MaxLight550
Note
Preservative Free
Applications Tested/Suitable for anti-OSF-2 antibody
Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-OSF-2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-OSF-2 antibody

Western Blot analysis in MCF-7 (lane 1), A375 (lane 2) cell line lysates (35ug/lane) using O8057-07. This demonstrates that O8057-07 detected the Osteoblast Specific Factor 2 protein (arrow).
Rabbit Anti-OSF-2 Polyclonal Antibody – Cat# MBS6491185

Western Blot (WB) of anti-OSF-2 antibody

Western Blot using O8057-07 (1:1000) Lane 1: human breast lysate Lane 2: rat lung lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP)Goat Anti-Rabbit at 1:10,000. Predicted band size: 93kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-OSF-2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6491185

Immunohistochemistry (IHC) of anti-OSF-2 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue using O8057-07 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of O8057-07 for immunohistochemistry.
Rabbit Anti-OSF-2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6491185

Immunofluorescence (IF) of anti-OSF-2 antibody

Confocal Immunofluorescence analysis of in A375 cells using O8057-07 followed by Alexa Fuor 488-conjugated goat anti-rabbit IgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-OSF-2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6491185

Product References and Citations for anti-OSF-2 antibody

1. Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A. J Bone Miner Res. 1999; 14: 1239-49. 2. Takeshita S, Kikuno R, Tezuka K, Amann E. Biochem J. 1993; 294 (Pt1): 271-8. 3. Ulstrup JC, Jeansson S, Wiker HG, Harboe M. Infect Immun. 1995; 63: 672-5.

Other Notes

Small volumes of anti-OSF-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OSF-2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
periostin isoform 1
NCBI Official Synonym Full Names
periostin, osteoblast specific factor
NCBI Official Symbol
POSTN
NCBI Official Synonym Symbols
PN; OSF2; OSF-2; PDLPOSTN; periostin
UniProt Protein Name
Periostin
UniProt Synonym Protein Names
Osteoblast-specific factor 2
Protein Family
UniProt Gene Name
POSTN
UniProt Synonym Gene Names
OSF2; PN; OSF-2
UniProt Entry Name
POSTN_HUMAN
NCBI Protein Information
periostin; osteoblast specific factor 2 (fasciclin I-like); periodontal ligament-specific periostin

UniProt Comments for OSF-2

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; trans-Golgi network

Molecular Function: heparin binding; protein binding; cell adhesion molecule binding

Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000