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Adipophilin (PLIN2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS617073

Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a… More details

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Product Overview

Online Datasheet in PDF
Product Name
Adipophilin (PLIN2), Polyclonal Antibody
Full Product Name
Adipophilin (ADFP, Adipose Differentiation-related Protein)
Product Gene Name
Product Synonym Names
Anti -Adipophilin (ADFP, Adipose Differentiation-related Protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.05 sodium azide, 30% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Adipophilin at ~48kD. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
48,075 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (19115759..19127573, complement). Location: 9p22.1
OMIM
103195
3D Structure
ModBase 3D Structure for Q99541
Immunogen
A synthetic peptide corresponding to an internal portion of human ADFP, within aa150-250 (Q99541). Cellular Localization: Membrane, Cytoplasm. Species sequence homology: human, monkey, porcine, and bovine 100%, mouse 94%, rat 84%.
Positive Control
Human liver lysate and mouse liver tissue.

Product Categories/Family for anti-PLIN2 antibody

Applications Tested/Suitable for anti-PLIN2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PLIN2 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Frozen): 1:200
Immunohistochemistry (Paraffin-embedded): 1:200 HIER using sodium citrate buffer recommended.
Western Blot: 2ug/ml

Western Blot (WB) of anti-PLIN2 antibody

Western Blot Analysis: Human ADFP in human liver lysate using MBS617073.
Rabbit Anti-PLIN2 Polyclonal Antibody – Cat# MBS617073

Immunohistochemistry (IHC) of anti-PLIN2 antibody

Immunohistochemistry Analysis: Paraffin-embedded mouse liver using MBS617073 followed by DAB with hematoxylin counterstain.
Rabbit Anti-PLIN2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS617073

Other Notes

Small volumes of anti-PLIN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLIN2 purchase

MBS617073 is an antibody targeting PLIN2. Also known as Adipophilin (ADFP, Adipose Differentiation-related Protein). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PLIN2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
adipophilin
NCBI Official Synonym Full Names
perilipin 2
NCBI Official Symbol
PLIN2
NCBI Official Synonym Symbols
ADFP; ADRP; MGC10598
UniProt Protein Name
Perilipin-2
UniProt Synonym Protein Names
Adipophilin; Adipose differentiation-related protein
Protein Family
UniProt Gene Name
PLIN2
UniProt Synonym Gene Names
ADFP
UniProt Entry Name
PLIN2_HUMAN
NCBI Protein Information
perilipin-2; adipophilin; OTTHUMP00000021107; adipose differentiation-related protein

NCBI Summary for PLIN2

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
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UniProt Comments for PLIN2

perilipin 2: May be involved in development and maintenance of adipose tissue. Belongs to the perilipin family.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: endoplasmic reticulum; plasma membrane; extracellular region; lipid particle; nucleus; cytosol

Biological Process: response to drug; long-chain fatty acid transport; sequestering of lipid; cellular lipid metabolic process; response to organic cyclic substance
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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