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Connexin 32 (GJB1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS614223

MBS614223 is an antibody targeting GJB1. Also known as Anti - Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein). Reactive in Rat. Host: RabbitSource: Rat. More details

$905 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Connexin 32 (GJB1), Polyclonal Antibody
Full Product Name
Anti - Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)
Product Gene Name
Product Synonym Names
Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)
Clonality
Polyclonal
Isotype
IgG
Host
Host: Rabbit
Source: Rat
Species Reactivity
Rat
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Purity/Purification
Serum
Specificity
Rat Cx32 immunogenic peptide sequences is the same in mouse, rat and human (4
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
32,025 Da
Chromosome Location
Chromosome: X; NC_000023.10 (70435062..70445065). Location: Xq13.1
OMIM
145900
3D Structure
ModBase 3D Structure for P08034
Immunogen
A 19 amino acid peptide sequence within the 2nd cytoplasmic domain of the rat Cx32 (2) was used to generate anti-rat Cx32. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)

Product Categories/Family for anti-GJB1 antibody

Applications Tested/Suitable for anti-GJB1 antibody
ELISA, Western Blot
Application Notes for anti-GJB1 antibody
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against this epitope has been used for Westerns (3) to detect a broad band of 28-30kD. Additional high MW. bands (aggregated Cx32) may also be detected.

Product References and Citations for anti-GJB1 antibody

• 1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; White, WT et al (1995) Kidney Intl. 48, 1148-1157; Evans, HW (1994) Biochem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-1942. Paul D (1986) J Cell Biol. 103, 123-134; Heynkes, R et al (1986) FEBS Lett. 205, 56-60; Nicholson, B (1985) JBC 260, 6514; Zimmer, DB et al (1987) JBC 262, 7751; Rahman, S et al (1991) J Cell Sci. 100, 567-5783. Zhang, J-T. and Nicholson, BJ (194) J. Membrane Biol. 139, 15-294. Nishi M et al (1991) Dev. Biol. 146, 117-130; Kumar, NM (1986) J Cell Biol. 103, 767-776

Other Notes

Small volumes of anti-GJB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GJB1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
connexin 32
NCBI Official Synonym Full Names
gap junction protein, beta 1, 32kDa
NCBI Official Symbol
GJB1
NCBI Official Synonym Symbols
CMTX; CX32; CMTX1
UniProt Protein Name
Gap junction beta-1 protein
UniProt Synonym Protein Names
Connexin-32; Cx32; GAP junction 28 kDa liver protein
UniProt Gene Name
GJB1
UniProt Synonym Gene Names
CX32
UniProt Entry Name
CXB1_HUMAN
NCBI Protein Information
gap junction beta-1 protein; connexin 32; connexin-32; OTTHUMP00000023502; OTTHUMP00000023503; OTTHUMP00000023504; GAP junction 28 kDa liver protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GJB1 antibody

 Reference Product  PubMed Publications
 GJB2 antibody  >11 publications with GJB1 and GJB2
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