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NR1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6490467

MBS6490467 is an antibody targeting NR1. Also known as NR1, Phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor) (HRP). Reactive in Rat. Host: Rabbit. More details

$1035 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NR1, Polyclonal Antibody
Full Product Name
NR1, Phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor) (HRP)
Product Gene Name
anti-NR1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by affinity chromatography.
Specificity
Recognizes rat NR1 when phosphorylated at Ser896. Species sequence homology: human, mouse 100%.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
130
3D Structure
ModBase 3D Structure for P35439
Immunogen
Synthetic peptide corresponding to rat NR1 phosphorylated at Ser896 (KLH). Epitope: Phosphorylated Ser896
Conjugate
HRP
Positive Control
Lambda phosphatase untreated and treated rat microsomal brain tissue lysates
Antigen Modification
Phosphorylated
Note
Preservative Free

Product Categories/Family for anti-NR1 antibody

Antibodies; Ion Channel
Applications Tested/Suitable for anti-NR1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-NR1 antibody
IHC: 1:25 detects NR1 in normal rat hippocampus tissue.
WB: 1ug/ml detects NR1 on 10ug of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-NR1 antibody

Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded normal rat hippocampus tissue (Fig. 1 & 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. In Fig. 1, N5380-08was incubated with phospho peptide and in Fig. 2, MBS626374 was incubated with the control peptide. Immunostaining was performed using a 1:25 dilution of MBS626374 Reactivity was detected using the IHC-Select Detection Kit. The staining pattern appears as cytoplasmic (Fig. 2). The Pyramidal layer of the hippocampus stains as expected. This lot is diffusive at higher titers, specifically in the cerebellum and fimbria of the hippocampus.
Rabbit Anti-NR1 Polyclonal Antibody – Cat# MBS6490467

Western Blot (WB) of anti-NR1 antibody

Western Blotting Analysis: Representative lot data. Lambda phosphatase untreated and treated rat microsomal brain tissue lysates were probed with MBS626374 (1ug/mL); untreated (lane 1) and treated (lane 2). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NR1 (~130 kDa).
Rabbit Anti-NR1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6490467

Other Notes

Small volumes of anti-NR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NR1. It may not necessarily be applicable to this product.

NCBI GI #
8393484
NCBI GeneID
24408
NCBI Accession #
NP_058706
UniProt Primary Accession #
P35439
UniProt Related Accession #
P35439
GenBank Nucleotide #
NP_058706.1
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 1 isoform 1a
NCBI Official Synonym Full Names
glutamate ionotropic receptor NMDA type subunit 1
NCBI Official Symbol
Grin1
NCBI Official Synonym Symbols
NR1; GluN1; NMDAR1
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 1
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1
Protein Family
Malate dehydrogenase
UniProt Gene Name
Grin1
UniProt Synonym Gene Names
Nmdar1; GluN1; NMD-R1
NCBI Protein Information
glutamate receptor ionotropic, NMDA 1

NCBI Summary for NR1

subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]

UniProt Comments for NR1

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
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Research Articles on NR1

1. In aged rats, overall decreases in NR1, NR2A, and NR2B expression and reduction in neuronal and endothelial NOS activities in the rat cerebral cortex were found. The age-dependent changes in NR1 and NR2B correlated with neuronal NOS in both hemispheres. The effect of sleep deprivation was also studied.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NR1 antibody

 Reference Product  PubMed Publications
 Drd1a antibody  >1 publications with NR1 and Drd1a

Pathways associated with anti-NR1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram

Diseases associated with anti-NR1 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >32 publications with NR1 and Mental Disorders
 Schizophrenia Antibodies  >25 publications with NR1 and Schizophrenia
 Learning Disorders Antibodies  >4 publications with NR1 and Learning Disorders
 Nerve Degeneration Antibodies  >3 publications with NR1 and Nerve Degeneration
 Neurodegenerative Diseases Antibodies  >3 publications with NR1 and Neurodegenerative Diseases
 Substance-Related Disorders Antibodies  >3 publications with NR1 and Substance-Related Disorders
 Seizures Antibodies  >3 publications with NR1 and Seizures
 Death Antibodies  >3 publications with NR1 and Death
 Neoplasms Antibodies  >3 publications with NR1 and Neoplasms
 Attention Deficit Disorder with Hyperactivity Antibodies  >2 publications with NR1 and Attention Deficit Disorder with Hyperactivity

Organs/Tissues associated with anti-NR1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >32 publications with NR1 and Brain
 Eye Antibodies  >2 publications with NR1 and Eye
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