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SGLT-2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS617689

MBS617689 is an antibody targeting SGLT-2. Also known as Anti - SGLT-2 (Sodium Glucose Transporter 2). Reactive in Rat. Host: RabbitSource: Rat. More details

$905 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SGLT-2, Polyclonal Antibody
Full Product Name
Anti - SGLT-2 (Sodium Glucose Transporter 2)
Product Gene Name
anti-SGLT-2 antibody
Product Synonym Names
SGLT-2 (Sodium Glucose Transporter 2)
Clonality
Polyclonal
Isotype
IgG
Host
Host: Rabbit
Source: Rat
Species Reactivity
Rat
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Purity/Purification
Serum
Specificity
The rat peptide sequence is ~67% conserved in mouse SGLT2. No significant sequence homology exists with other SGLTs. For human SGLT2, we recommend the use antibody that is made to the human SGLT-2 peptide. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 16 AA peptide sequence from the N-terminal, cytoplasmic domain 1 of Rat SGLT-2 (1) was synthesized, coupled to KLH

Product Categories/Family for anti-SGLT-2 antibody

Abs to Glucose Transporters
Applications Tested/Suitable for anti-SGLT-2 antibody
ELISA, Western Blot
Application Notes for anti-SGLT-2 antibody
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 (ECL). A band of 95-110kD has been detected in brush border membrane (2).
ELISA:1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Product References and Citations for anti-SGLT-2 antibody

• 1. Wells RG et al (1995) JBC 270, 29365-29371; Wells RG et al (1992) Amj. J. Physiol. 263, F459-F465; Kanai Y et al (1994) J. Clin. Invest. 93, 397-404; Wright E (2001) Am. J. Physiol. Renal Physiol. 280, F10-F18 (review)

Other Notes

Small volumes of anti-SGLT-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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