Goat Anti-SLC1A3 Polyclonal Antibody – Cat# MBS423166

(2ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EAAT1/GLAST1 (SLC1A3) Antibody – Goat Polyclonal

Name: SLC1A3 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423166
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423166

MBS423166 is an antibody targeting SLC1A3. Also known as Goat anti-EAAT1/GLAST1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EAAT1/GLAST1 (SLC1A3), Polyclonal Antibody

Full Product Name

Goat anti-EAAT1/GLAST1 Antibody

Product Gene Name

anti-SLC1A3 antibody

Product Synonym Gene Name

EAAT1; GLAST1

Product Synonym Names

Goat Anti-EAAT1 / GLAST1 (aa197-208) Antibody; SLC1A3; solute carrier family 1 (glial high affinity glutamate transporter), member 3; EA6; EAAT1; FLJ25094; GLAST; GLAST1; GLAST-1; OTTHUMP00000116002; excitatory amino acid transporter 1; sodium-dependent glutamate/aspartate transporter 1; solute carrie; SLC1A3 antibody; solute carrier family 1 (glial high affinity glutamate transporter); member 3 antibody; EA6 antibody; EAAT1 antibody; FLJ25094 antibody; GLAST antibody; GLAST1 antibody; GLAST-1 antibody; OTTHUMP00000116002 antibody; excitatory amino acid transporter 1 antibody; sodium-dependent glutamate/aspartate transporter 1 antibody; solute carrier family 1 member 3 antibody; EAAT1 / GLAST1

Immunogen Sequence

DNETEKPIDS ETK

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

EAAT1 / GLAST1 peptide (MBS427921) is used for blocking the activity of EAAT1/GLAST1 antibody (MBS423166)

Molecular Weight

55,149 Da

OMIM

600111

Immunogen

Peptide with sequence C-DNETEKPIDSETK, from the C Terminus of the protein sequence according to NP_004163.3; NP_001160167.1.

Epitope

C Terminus

Note

This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-SLC1A3 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-SLC1A3 antibody

Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 60kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-SLC1A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC1A3. It may not necessarily be applicable to this product.

NCBI GI #

169790839

NCBI GeneID

6507

NCBI Accession #

NP_004163.3

NCBI Related Accession #

NP_001160167.1

UniProt Secondary Accession #

Q4JCQ8; B2R5T3

UniProt Related Accession #

P43003

GenBank Nucleotide #

NM_004172.4

NCBI Official Full Name

excitatory amino acid transporter 1 isoform 1

NCBI Official Synonym Full Names

solute carrier family 1 member 3

NCBI Official Symbol

SLC1A3

NCBI Official Synonym Symbols

EA6; EAAT1; GLAST; GLAST1

UniProt Protein Name

Excitatory amino acid transporter 1

UniProt Synonym Protein Names

Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3

Protein Family

Excitatory amino acid transporter

UniProt Gene Name

SLC1A3

UniProt Synonym Gene Names

EAAT1; GLAST; GLAST1; GLAST-1

UniProt Entry Name

EAA1_HUMAN

NCBI Protein Information

excitatory amino acid transporter 1

NCBI Summary for SLC1A3

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]

UniProt Comments for SLC1A3

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: basolateral plasma membrane; cell soma; cell surface; dendritic spine; fibril; integral to membrane; membrane; mitochondrial inner membrane; plasma membrane

Molecular Function: amino acid transmembrane transporter activity; glutamate binding; high-affinity glutamate transmembrane transporter activity; L-glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity

Biological Process: auditory behavior; cranial nerve development; gamma-aminobutyric acid biosynthetic process; glutamate biosynthetic process; glutamate secretion; ion transport; L-glutamate import; malate-aspartate shuttle; neuromuscular process controlling balance; neuron morphogenesis during differentiation; neurotransmitter uptake; positive regulation of defense response to virus by host; positive regulation of synaptic transmission; response to antibiotic; response to drug; response to light stimulus; response to wounding; sensory perception of sound; synaptic transmission

Disease: Episodic Ataxia, Type 6

Research Articles on SLC1A3

1. There was no association between pyramidal cell EAAT1 splice variant expression and schizophrenia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC1A3 antibody

Reference Product	PubMed Publications
GLUL antibody	>6 publications with SLC1A3 and GLUL
FABP7 antibody	>4 publications with SLC1A3 and FABP7
GDNF antibody	>4 publications with SLC1A3 and GDNF
EGFR antibody	>3 publications with SLC1A3 and EGFR
SLC6A1 antibody	>3 publications with SLC1A3 and SLC6A1
SGK1 antibody	>1 publications with SLC1A3 and SGK1

Pathways associated with anti-SLC1A3 antibody

Products by Pathway	Pathway Diagram
Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway antibodies	Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway Diagram
Glutamate Neurotransmitter Release Cycle Pathway antibodies	Glutamate Neurotransmitter Release Cycle Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Neurotransmitter Release Cycle Pathway antibodies	Neurotransmitter Release Cycle Pathway Diagram
Neurotransmitter Uptake And Metabolism In Glial Cells Pathway antibodies	Neurotransmitter Uptake And Metabolism In Glial Cells Pathway Diagram
SIDS Susceptibility Pathways antibodies	SIDS Susceptibility Pathways Diagram
SLC-mediated Transmembrane Transport Pathway antibodies	SLC-mediated Transmembrane Transport Pathway Diagram
Synaptic Vesicle Pathway antibodies	Synaptic Vesicle Pathway Diagram

Diseases associated with anti-SLC1A3 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>123 publications with SLC1A3 and Nervous System Diseases
Brain Diseases Antibodies	>89 publications with SLC1A3 and Brain Diseases
Disease Models, Animal Antibodies	>59 publications with SLC1A3 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>34 publications with SLC1A3 and Cardiovascular Diseases
Epilepsy Antibodies	>22 publications with SLC1A3 and Epilepsy
Seizures Antibodies	>15 publications with SLC1A3 and Seizures
Nerve Degeneration Antibodies	>14 publications with SLC1A3 and Nerve Degeneration
Inflammation Antibodies	>12 publications with SLC1A3 and Inflammation
Movement Disorders Antibodies	>12 publications with SLC1A3 and Movement Disorders
Depressive Disorder Antibodies	>10 publications with SLC1A3 and Depressive Disorder

Organs/Tissues associated with anti-SLC1A3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>279 publications with SLC1A3 and Brain
Nerve Antibodies	>79 publications with SLC1A3 and Nerve
Eye Antibodies	>51 publications with SLC1A3 and Eye
Blood Antibodies	>37 publications with SLC1A3 and Blood
Spinal Cord Antibodies	>36 publications with SLC1A3 and Spinal Cord
Embryonic Tissue Antibodies	>20 publications with SLC1A3 and Embryonic Tissue
Muscle Antibodies	>14 publications with SLC1A3 and Muscle
Kidney Antibodies	>13 publications with SLC1A3 and Kidney
Bone Antibodies	>7 publications with SLC1A3 and Bone
Heart Antibodies	>7 publications with SLC1A3 and Heart