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VIME (VIM) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9211419

VIME is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. This protein is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing… More details

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Product Overview

Online Datasheet in PDF
Product Name
VIME, Polyclonal Antibody
Full Product Name
VIME Antibody
Product Gene Name
anti-VIM antibody
Product Synonym Names
Vimentin; VIM
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.5 (lot specific)
Specificity
This Vimentin antibody is generated from rabbits immunized with Vimentin recombinant protein.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
53652
OMIM
116300
3D Structure
ModBase 3D Structure for P08670
Antigen Type
Recombinant Protein
Antigen Source
HUMAN

Product Categories/Family for anti-VIM antibody

Cancer; Developmental biology; Neuroscience; Signal transduction
Applications Tested/Suitable for anti-VIM antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-VIM antibody
WB~~1:1000

Western Blot (WB) of anti-VIM antibody

Western blot analysis of VIME Antibody in A375 cell line lysates (35ug/lane). VIME (arrow) was detected using the purified Pab.
Rabbit Anti-VIM Polyclonal Antibody – Cat# MBS9211419

Immunohistochemistry (IHC) of anti-VIM antibody

VIME Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VIME Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-VIM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9211419

Flow Cytometry (FC/FACS) of anti-VIM antibody

VIME Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-VIM Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9211419

Product References and Citations for anti-VIM antibody

Morishima,N., Genes Cells 4 (7), 401-414 (1999)

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS9211419 is an antibody targeting VIM. Also known as VIME Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Primary Accession #
P08670
UniProt Secondary Accession #
Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.3
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
HEL113; CTRCT30
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
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UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; leading edge; cytoplasm; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30
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Research Articles on VIM

1. Authors propose a model whereby vimentin promotes FAK stabilization through VAV2-mediated Rac1 activation. This model may explain why vimentin expressing metastatic lung cancer cells are more motile and invasive.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-VIM antibody

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Diseases associated with anti-VIM antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >428 publications with VIM and Nervous System Diseases
 Neurodegenerative Diseases Antibodies  >180 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >60 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >42 publications with VIM and Kidney Diseases
 Disease Models, Animal Antibodies  >40 publications with VIM and Disease Models, Animal
 Pain Antibodies  >38 publications with VIM and Pain
 Hemorrhage Antibodies  >36 publications with VIM and Hemorrhage
 Neoplasm Metastasis Antibodies  >35 publications with VIM and Neoplasm Metastasis
 Liver Diseases Antibodies  >35 publications with VIM and Liver Diseases
 Breast Neoplasms Antibodies  >32 publications with VIM and Breast Neoplasms

Organs/Tissues associated with anti-VIM antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >515 publications with VIM and Brain
 Blood Antibodies  >222 publications with VIM and Blood
 Muscle Antibodies  >122 publications with VIM and Muscle
 Nerve Antibodies  >85 publications with VIM and Nerve
 Bone Antibodies  >83 publications with VIM and Bone
 Kidney Antibodies  >65 publications with VIM and Kidney
 Liver Antibodies  >58 publications with VIM and Liver
 Bone Marrow Antibodies  >54 publications with VIM and Bone Marrow
 Eye Antibodies  >48 publications with VIM and Eye
 Lung Antibodies  >46 publications with VIM and Lung
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