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Vimentin (VIM) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6505762

MBS6505762 is an antibody targeting VIM. Also known as Vimentin (S82) (VIM) (APC). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Polyclonal Antibody
Full Product Name
Vimentin (S82) (VIM) (APC)
Product Gene Name
anti-VIM antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human VIM.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
53,652 Da
OMIM
193060
3D Structure
ModBase 3D Structure for P08670
Immunogen
Synthetic peptide corresponding to amino acid residues surrounding S82 of human VIM (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-VIM antibody

Antibodies; Extracellular Matrix Proteins
Applications Tested/Suitable for anti-VIM antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-VIM antibody
IHC: 1:10-1:50
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-VIM antibody

Fluorescent confocal image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2739a Vimentin (S82) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data.
Rabbit Anti-VIM Polyclonal Antibody – Cat# MBS6505762

Western Blot (WB) of anti-VIM antibody

Western blot analysis of Vimentin-S82 in Hela cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.
Rabbit Anti-VIM Polyclonal Antibody, Western Blot (WB) – Cat# MBS6505762

Western Blot (WB) of anti-VIM antibody

Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
Rabbit Anti-VIM Polyclonal Antibody, Western Blot (WB) – Cat# MBS6505762

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-VIM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6505762

Product References and Citations for anti-VIM antibody

1. Whipple, R.A., Cancer Res. 68(14), 5678-5688 (2008). 2. Garcia-Verdugo, I., Biochemistry 47(18), 5127-5138 (2008). 3. Merdes, A., J. Cell Biol. 115(2), 397-410 (1991).

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371
UniProt Primary Accession #
P08670
UniProt Secondary Accession #
Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.4
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
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UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30
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Research Articles on VIM

1. Vimentin and its interaction with Shigella flexneri IpaC are dispensable for effector translocation pore formation, but are required for stable docking of Shigella flexneri to cells; moreover, stable docking triggers effector secretion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-VIM antibody

 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies  Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram

Diseases associated with anti-VIM antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >383 publications with VIM and Nervous System Diseases
 Neoplasms Antibodies  >283 publications with VIM and Neoplasms
 Brain Diseases Antibodies  >246 publications with VIM and Brain Diseases
 Neurodegenerative Diseases Antibodies  >166 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >46 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >37 publications with VIM and Kidney Diseases
 Hemorrhage Antibodies  >32 publications with VIM and Hemorrhage
 Disease Models, Animal Antibodies  >32 publications with VIM and Disease Models, Animal
 Pain Antibodies  >31 publications with VIM and Pain
 Liver Diseases Antibodies  >29 publications with VIM and Liver Diseases

Organs/Tissues associated with anti-VIM antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >461 publications with VIM and Brain
 Blood Antibodies  >187 publications with VIM and Blood
 Muscle Antibodies  >101 publications with VIM and Muscle
 Nerve Antibodies  >79 publications with VIM and Nerve
 Bone Antibodies  >69 publications with VIM and Bone
 Kidney Antibodies  >52 publications with VIM and Kidney
 Liver Antibodies  >51 publications with VIM and Liver
 Bone Marrow Antibodies  >46 publications with VIM and Bone Marrow
 Eye Antibodies  >38 publications with VIM and Eye
 Vascular Antibodies  >35 publications with VIM and Vascular
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