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Casein Kinase 1 Substrate (CK1) Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635174

Synthetic peptide based around an Inhibitor-2 peptide sequence that is phosphorylated by Casein Kinase 1 (RRKDLHDDEEDEAMSITA) (linear). Two amino acid substitutions have been made to render the peptide refractory to Casein Kinase 2. The substitutions, which… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Casein Kinase 1 Substrate (CK1), Protein
Full Product Name
Casein Kinase 1 Substrate
Product Gene Name
Host
Synthetic peptide
Form/Format
Supplied as a liquid; 1mg packaged in two vials, each vial containing 500ug peptide in 100ul sterile, distilled water (2.3mM).
Purity/Purification
Highly Purified
~95%
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Stable for 12 months at -20 degree C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
50,947 Da
Chromosome Location
Chromosome: 4; NC_003075.7 (13227450..13230797, complement). Location: chromosome: 4
3D Structure
ModBase 3D Structure for P42158

Product Categories/Family for CK1 protein

Application Notes for CK1 protein
Suitable for use in Kinase assay.
Dilution: Protein Kinase Assay: 250uM of this lot of substrate peptide was phosphorylated by a 1:100 dilution of Casein Kinase 1d (aa 1-294), active.

Other Notes

Small volumes of CK1 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CK1 purchase

MBS635174 is a protein targeting CK1. Also known as Casein Kinase 1 Substrate. Host: Synthetic peptide.

Additional Details

NCBI/Uniprot data below describe general gene information for CK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
casein kinase 1
NCBI Official Symbol
CK1
NCBI Official Synonym Symbols
casein kinase 1; CK1; CKL1
UniProt Protein Name
Casein kinase I isoform delta-like
UniProt Gene Name
CKI-delta
UniProt Synonym Gene Names
CKI-delta
UniProt Entry Name
KC1D_ARATH
NCBI Protein Information
casein kinase 1

NCBI Summary for CK1

Encodes a member of the casein kinase 1 protein family that is expressed in punctate particles at the cell periphery suggesting possible plasmodesmatal localization.

UniProt Comments for CK1

Function: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Monomer

By similarity.

Subcellular location: Cytoplasm

By similarity.

Post-translational modification: Autophosphorylated

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence AAA50233.1 differs from that shown. Reason: Frameshift at positions 381 and 414.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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