Figure 1. Elisa plates were pre-coated with DYKDDDDK Tag LGR5-Nanodisc (0.2ug/per well). Serial diluted anti-DYKDDDDK monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-DYKDDDDK monoclonal antibody binding with LGR5-Nanodisc is 5.199ng/ml.

LGR5 Human Protein

Name: LGR5 Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS189830
Price: 1320 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS189830

The protein is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This… More details

$1320 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LGR5, Protein

Full Product Name

Human LGR5 full length protein-synthetic nanodisc

Product Gene Name

LGR5 protein

Product Synonym Names

FEX; GPR49; GPR67; GRP49; HG38

Host

HEK293

Form/Format

Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.

Preparation and Storage

Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing).

Molecular Weight

99998 MW

OMIM

AF062006 mRNA

3D Structure

ModBase 3D Structure for O75473

Product Categories/Family for LGR5 protein

FLP 489; Full Length Transmembrane Proteins

Other Notes

Small volumes of LGR5 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGR5 purchase

MBS189830 is a protein targeting LGR5. Also known as Human LGR5 full length protein-synthetic nanodisc. Host: HEK293.

Additional Details

NCBI/Uniprot data below describe general gene information for LGR5. It may not necessarily be applicable to this product.

NCBI GI #

469608414

NCBI GeneID

8549

NCBI Accession #

NP_001264155.1

UniProt Primary Accession #

O75473

UniProt Secondary Accession #

Q4VAM0; Q4VAM2; Q9UP75; D8MCT0

UniProt Related Accession #

O75473

GenBank Nucleotide #

NM_001277226.1

NCBI Official Full Name

leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2

NCBI Official Synonym Full Names

leucine rich repeat containing G protein-coupled receptor 5

NCBI Official Symbol

LGR5

NCBI Official Synonym Symbols

FEX; HG38; GPR49; GPR67; GRP49

UniProt Protein Name

Leucine-rich repeat-containing G-protein coupled receptor 5

UniProt Synonym Protein Names

G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38

Protein Family

Leucine-rich repeat-containing G-protein coupled receptor

UniProt Gene Name

LGR5

UniProt Synonym Gene Names

GPR49; GPR67

NCBI Protein Information

leucine-rich repeat-containing G-protein coupled receptor 5

NCBI Summary for LGR5

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

UniProt Comments for LGR5

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Research Articles on LGR5

1. This study uncovers a novel cross-talk between LGR5 and TGFbeta signaling in colon cancer and identifies LGR5 as a new modulator of TGFbeta signaling able to suppress colon cancer metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LGR5 protein

Reference Product	PubMed Publications
OLFM4 protein	>18 publications with LGR5 and OLFM4
DCLK1 protein	>16 publications with LGR5 and DCLK1
TSPAN8 protein	>13 publications with LGR5 and TSPAN8
JAZF1 protein	>13 publications with LGR5 and JAZF1
ADAMTS9 protein	>11 publications with LGR5 and ADAMTS9
CAMK1D protein	>10 publications with LGR5 and CAMK1D
THADA protein	>10 publications with LGR5 and THADA
CDC123 protein	>10 publications with LGR5 and CDC123
MBD3 protein	>1 publications with LGR5 and MBD3

Pathways associated with LGR5 protein

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies	RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By WNT In Cancer Pathway antibodies	Signaling By WNT In Cancer Pathway Diagram
Signaling By Wnt Pathway antibodies	Signaling By Wnt Pathway Diagram
TCF Dependent Signaling In Response To WNT Pathway antibodies	TCF Dependent Signaling In Response To WNT Pathway Diagram
XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies	XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram
Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies	Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
Regulation Of FZD By Ubiquitination Pathway antibodies	Regulation Of FZD By Ubiquitination Pathway Diagram

Diseases associated with LGR5 protein

Disease Name	Pubmed Publications
Colonic Neoplasms Antibodies	>51 publications with LGR5 and Colonic Neoplasms
Cell Transformation, Neoplastic Antibodies	>30 publications with LGR5 and Cell Transformation, Neoplastic
Inflammation Antibodies	>25 publications with LGR5 and Inflammation
Neoplasm Metastasis Antibodies	>24 publications with LGR5 and Neoplasm Metastasis
Liver Diseases Antibodies	>18 publications with LGR5 and Liver Diseases
Liver Neoplasms Antibodies	>18 publications with LGR5 and Liver Neoplasms
Disease Models, Animal Antibodies	>15 publications with LGR5 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>13 publications with LGR5 and Neoplasms, Experimental
Precancerous Conditions Antibodies	>10 publications with LGR5 and Precancerous Conditions
Necrosis Antibodies	>8 publications with LGR5 and Necrosis

Organs/Tissues associated with LGR5 protein

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>201 publications with LGR5 and Intestine
Liver Antibodies	>45 publications with LGR5 and Liver
Stomach Antibodies	>43 publications with LGR5 and Stomach
Skin Antibodies	>28 publications with LGR5 and Skin
Embryonic Tissue Antibodies	>25 publications with LGR5 and Embryonic Tissue
Bone Antibodies	>20 publications with LGR5 and Bone
Brain Antibodies	>11 publications with LGR5 and Brain
Lymph Node Antibodies	>11 publications with LGR5 and Lymph Node
Ovary Antibodies	>11 publications with LGR5 and Ovary
Muscle Antibodies	>8 publications with LGR5 and Muscle