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Testosterone (HSD17B3) ELISA Kit for Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2600536

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rabbit Testo monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and… More details

$370 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Testosterone (HSD17B3), ELISA Kit
Full Product Name
Rabbit Testosterone ELISA Kit
Product Gene Name
HSD17B3 elisa kit
Species Reactivity
Rabbit
Assay Type
Sandwich
Samples
Rabbit serum, plasma or cell culture supernatant and organizations in the natural and recombinant Testo concentration.
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
The minimum detectable Rabbit Testo up to 0.05 ng/ml.
Specificity
No cross-reaction with other factors.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
34,516 Da
OMIM
605573
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of HSD17B3 elisa kit

Rabbit HSD17B3 ELISA Kit – Cat# MBS2600536

Other Notes

Small volumes of HSD17B3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSD17B3 purchase

MBS2600536 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Testosterone (HSD17B3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSD17B3. The ELISA analytical biochemical technique of the MBS2600536 kit is based on HSD17B3 antibody-HSD17B3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD17B3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD17B3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HSD17B3. It may not necessarily be applicable to this product.

NCBI GI #
4557649
NCBI GeneID
3293
NCBI Accession #
NP_000188.1
UniProt Related Accession #
P37058
GenBank Nucleotide #
NM_000197.1
NCBI Official Full Name
testosterone 17-beta-dehydrogenase 3
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 3
NCBI Official Symbol
HSD17B3
NCBI Official Synonym Symbols
EDH17B3; SDR12C2
UniProt Protein Name
Testosterone 17-beta-dehydrogenase 3
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
Protein Family
Testosterone 17-beta-dehydrogenase
UniProt Gene Name
HSD17B3
UniProt Synonym Gene Names
EDH17B3; 17-beta-HSD 3
UniProt Entry Name
DHB3_HUMAN
NCBI Protein Information
testosterone 17-beta-dehydrogenase 3; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2

NCBI Summary for HSD17B3

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]
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UniProt Comments for HSD17B3

HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Oxidoreductase; EC 1.1.1.64; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity

Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process

Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency
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Research Articles on HSD17B3

1. These results suggest that the HSD17B3 G289S polymorphism may be a potential risk modifier for hypospadias.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HSD17B3 elisa kit

 Reference Product  PubMed Publications
 CYP17A1 elisa kit  >24 publications with HSD17B3 and CYP17A1
 SRD5A2 elisa kit  >15 publications with HSD17B3 and SRD5A2
 CYP19A1 elisa kit  >13 publications with HSD17B3 and CYP19A1
 HSD3B1 elisa kit  >12 publications with HSD17B3 and HSD3B1
 SRD5A1 elisa kit  >8 publications with HSD17B3 and SRD5A1
 HSD3B2 elisa kit  >5 publications with HSD17B3 and HSD3B2
 UGT2B17 elisa kit  >3 publications with HSD17B3 and UGT2B17
 UGT2B15 elisa kit  >3 publications with HSD17B3 and UGT2B15

Pathways associated with HSD17B3 elisa kit

 Products by Pathway  Pathway Diagram
 Androgen Biosynthesis Pathway antibodies  Androgen Biosynthesis Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Steroid Biosynthesis Pathway antibodies  Steroid Biosynthesis Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram

Diseases associated with HSD17B3 elisa kit

 Disease Name  Pubmed Publications
 Urogenital Abnormalities Antibodies  >21 publications with HSD17B3 and Urogenital Abnormalities
 Disorders of Sex Development Antibodies  >19 publications with HSD17B3 and Disorders of Sex Development
 Genital Diseases, Male Antibodies  >15 publications with HSD17B3 and Genital Diseases, Male
 Prostatic Diseases Antibodies  >10 publications with HSD17B3 and Prostatic Diseases
 Prostatic Neoplasms Antibodies  >9 publications with HSD17B3 and Prostatic Neoplasms
 Hyperplasia Antibodies  >4 publications with HSD17B3 and Hyperplasia
 Ovarian Diseases Antibodies  >3 publications with HSD17B3 and Ovarian Diseases
 Prenatal Exposure Delayed Effects Antibodies  >3 publications with HSD17B3 and Prenatal Exposure Delayed Effects
 Infertility, Male Antibodies  >3 publications with HSD17B3 and Infertility, Male
 17-Hydroxysteroid Dehydrogenase Deficiency Antibodies  >2 publications with HSD17B3 and 17-Hydroxysteroid Dehydrogenase Deficiency

Organs/Tissues associated with HSD17B3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >28 publications with HSD17B3 and Testis
 Brain Antibodies  >6 publications with HSD17B3 and Brain
 Placenta Antibodies  >2 publications with HSD17B3 and Placenta
 Uncharacterized Tissue Antibodies  >1 publications with HSD17B3 and Uncharacterized Tissue
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