Antithrombin (AT) ELISA Kit for Rat

Name: Rat AT ELISA Kit; Antithrombin; MyBioSource
Brand: MyBioSource
SKU: MBS2024778
Price: 455 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2024778

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Antithrombin (AT). Standards or samples are then added to the appropriate microtiter plate wells… More details

$455 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Antithrombin (AT), ELISA Kit

Full Product Name

Rat Antithrombin (AT) ELISA Kit

Product Gene Name

AT elisa kit

Product Synonym Names

AT3; ATIII; SERPINC1; Anti-Thrombin Antibodies; Serpin Peptidase Inhibitor Clade C Member 1; Coding Sequence Signal Peptide Antithrombin Part 1

Species Reactivity

Rat

Assay Type

Double-antibody Sandwich

Samples

Plasma

Detection Range

15.6-1,000ng/mL

Sensitivity

6.9ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Antithrombin (AT).
No significant cross-reactivity or interference between Antithrombin (AT) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

52,602 Da

OMIM

107300

3D Structure

ModBase 3D Structure for P01008

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Antithrombin (AT) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Antithrombin (AT) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for AT elisa kit

Enzyme & Kinase; Metabolic pathway; Hematology

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of AT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AT purchase

MBS2024778 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Antithrombin (AT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AT. The ELISA analytical biochemical technique of the MBS2024778 kit is based on AT antibody-AT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AT. It may not necessarily be applicable to this product.

NCBI GI #

28907

NCBI GeneID

462

NCBI Accession #

CAA48690.1

UniProt Primary Accession #

P01008

UniProt Secondary Accession #

P78439; P78447; Q13815; Q5TC78; Q7KZ43; Q7KZ97; Q9UC78; B2R6P0

UniProt Related Accession #

P01008

NCBI Official Full Name

antithrombin

NCBI Official Synonym Full Names

serpin peptidase inhibitor, clade C (antithrombin), member 1

NCBI Official Symbol

SERPINC1

NCBI Official Synonym Symbols

AT3; AT3D; ATIII; THPH7

UniProt Protein Name

Antithrombin-III

UniProt Synonym Protein Names

Serpin C1

UniProt Gene Name

SERPINC1

UniProt Synonym Gene Names

AT3; ATIII

UniProt Entry Name

ANT3_HUMAN

NCBI Protein Information

antithrombin-III

NCBI Summary for AT

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]

UniProt Comments for AT

SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protein binding; protease binding

Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient

Disease: Antithrombin Iii Deficiency

Research Articles on AT

1. We identified a novel hereditary mutation, g.1267G>A (p.A391T), in the AT gene, which reduces its heparin binding capacity and might be associated with resistance to heparin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with AT elisa kit

Products by Pathway	Pathway Diagram
Common Pathway antibodies	Common Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Glypican 1 Network Pathway antibodies	Glypican 1 Network Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Intrinsic Pathway antibodies	Intrinsic Pathway Diagram

Diseases associated with AT elisa kit

Disease Name	Pubmed Publications
Vascular Diseases Antibodies	>3837 publications with AT and Vascular Diseases
Thrombosis Antibodies	>3262 publications with AT and Thrombosis
Blood Coagulation Disorders Antibodies	>2707 publications with AT and Blood Coagulation Disorders
Antithrombin III Deficiency Antibodies	>1870 publications with AT and Antithrombin III Deficiency
Venous Thrombosis Antibodies	>1348 publications with AT and Venous Thrombosis
Thromboembolism Antibodies	>1093 publications with AT and Thromboembolism
Disseminated Intravascular Coagulation Antibodies	>895 publications with AT and Disseminated Intravascular Coagulation
Inflammation Antibodies	>736 publications with AT and Inflammation
Hemorrhage Antibodies	>620 publications with AT and Hemorrhage
Liver Diseases Antibodies	>519 publications with AT and Liver Diseases

Organs/Tissues associated with AT elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>879 publications with AT and Liver
Kidney Antibodies	>395 publications with AT and Kidney
Lung Antibodies	>351 publications with AT and Lung
Brain Antibodies	>248 publications with AT and Brain
Muscle Antibodies	>139 publications with AT and Muscle
Connective Tissue Antibodies	>41 publications with AT and Connective Tissue
Spleen Antibodies	>37 publications with AT and Spleen
Mammary Gland Antibodies	>3 publications with AT and Mammary Gland
Uncharacterized Tissue Antibodies	>1 publications with AT and Uncharacterized Tissue