Calbindin (CALB) ELISA Kit for Rat

Name: Rat CALB ELISA Kit; Calbindin; MyBioSource
Brand: MyBioSource
SKU: MBS2019483
Price: 455 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2019483

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calbindin (CALB). Standards or samples are then added to the appropriate microtiter plate wells… More details

$455 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Calbindin (CALB), ELISA Kit

Full Product Name

Rat Calbindin (CALB) ELISA Kit

Product Gene Name

CALB elisa kit

Product Synonym Names

CAB27; Calbindin 1,28kDa; Calbindin D28; Vitamin D-dependent calcium-binding protein, avian-type

Species Reactivity

Rat

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma, Tissue homogenates, Urine and other biological fluids

Detection Range

9.38-600pg/mL

Sensitivity

3.3pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Calbindin (CALB).
No significant cross-reactivity or interference between Calbindin (CALB) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

23,609 Da

OMIM

114050

3D Structure

ModBase 3D Structure for P05937

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Calbindin (CALB) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Calbindin (CALB) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for CALB elisa kit

Metabolic pathway

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of CALB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CALB purchase

MBS2019483 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calbindin (CALB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CALB. The ELISA analytical biochemical technique of the MBS2019483 kit is based on CALB antibody-CALB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CALB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CALB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CALB. It may not necessarily be applicable to this product.

NCBI GI #

4826655

NCBI GeneID

793

NCBI Accession #

NP_004920.1

UniProt Primary Accession #

P05937

UniProt Secondary Accession #

B2R696; B7Z9J4

UniProt Related Accession #

P05937

GenBank Nucleotide #

NM_004929.3

NCBI Official Full Name

calbindin

NCBI Official Synonym Full Names

calbindin 1, 28kDa

NCBI Official Symbol

CALB1

NCBI Official Synonym Symbols

CALB; D-28K

UniProt Protein Name

Calbindin

UniProt Synonym Protein Names

Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type

Protein Family

Coniferyl aldehyde dehydrogenase

UniProt Gene Name

CALB1

UniProt Synonym Gene Names

CAB27

UniProt Entry Name

CALB1_HUMAN

NCBI Protein Information

calbindin

NCBI Summary for CALB

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

UniProt Comments for CALB

CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: cell soma; axon; dendrite; synapse; nucleus; cytosol

Molecular Function: vitamin D binding; protein binding; zinc ion binding; calcium ion binding

Biological Process: cytosolic calcium ion homeostasis; long-term memory; short-term memory; regulation of synaptic plasticity; locomotory behavior

Research Articles on CALB

1. expressed by different neuron types, both inhibitory (GABAergic) and excitatory (glutamatergic), involved in both intrinsic and extrinsic circuits of the human cerebellar cortex

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CALB elisa kit

Reference Product	PubMed Publications
CA2 elisa kit	>107 publications with CALB and CA2
S100G elisa kit	>42 publications with CALB and S100G
TRPV5 elisa kit	>22 publications with CALB and TRPV5
TRPV6 elisa kit	>11 publications with CALB and TRPV6
BDNF elisa kit	>8 publications with CALB and BDNF
GDNF elisa kit	>4 publications with CALB and GDNF
PVALB elisa kit	>1 publications with CALB and PVALB

Pathways associated with CALB elisa kit

Products by Pathway	Pathway Diagram
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies	Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies	Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram

Diseases associated with CALB elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>115 publications with CALB and Nervous System Diseases
Brain Diseases Antibodies	>94 publications with CALB and Brain Diseases
Nerve Degeneration Antibodies	>47 publications with CALB and Nerve Degeneration
Epilepsy Antibodies	>27 publications with CALB and Epilepsy
Disease Models, Animal Antibodies	>24 publications with CALB and Disease Models, Animal
Seizures Antibodies	>18 publications with CALB and Seizures
Kidney Diseases Antibodies	>15 publications with CALB and Kidney Diseases
Neurotoxicity Syndromes Antibodies	>9 publications with CALB and Neurotoxicity Syndromes
Necrosis Antibodies	>9 publications with CALB and Necrosis
Cognition Disorders Antibodies	>8 publications with CALB and Cognition Disorders

Organs/Tissues associated with CALB elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>461 publications with CALB and Brain
Kidney Antibodies	>100 publications with CALB and Kidney
Blood Antibodies	>52 publications with CALB and Blood
Embryonic Tissue Antibodies	>35 publications with CALB and Embryonic Tissue
Muscle Antibodies	>23 publications with CALB and Muscle
Eye Antibodies	>17 publications with CALB and Eye
Lung Antibodies	>14 publications with CALB and Lung
Connective Tissue Antibodies	>6 publications with CALB and Connective Tissue
Stomach Antibodies	>5 publications with CALB and Stomach
Ear Antibodies	>4 publications with CALB and Ear