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CD86 ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS825025

Sandwich ELISA for quantitative detection of Rat CD86 concentrations. More details

$640 / 96 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD86, ELISA Kit
Full Product Name
Rat CD86 ELISA Kit
Product Gene Name
CD86 elisa kit
Product Synonym Names
CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Cell Culture Supernatants, Serum
Detection Range
62.5 pg/ml - 4000 pg/ml
Sensitivity
10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Molecular Weight
17,153 Da
3D Structure
ModBase 3D Structure for Q62810
Detection Method
Colorimetric
Applications Tested/Suitable for CD86 elisa kit
Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only) of CD86 elisa kit

Rat CD86 ELISA Kit – Cat# MBS825025

Other Notes

Small volumes of CD86 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD86 purchase

MBS825025 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD86, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD86. The ELISA analytical biochemical technique of the MBS825025 kit is based on CD86 antibody-CD86 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD86 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD86. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CD86. It may not necessarily be applicable to this product.

NCBI GI #
81863176
NCBI GeneID
12524
NCBI Accession #
Q62810
UniProt Primary Accession #
Q62810
UniProt Related Accession #
P42082
NCBI Official Full Name
B7-2
NCBI Official Synonym Full Names
CD86 antigen
NCBI Official Symbol
Cd86
NCBI Official Synonym Symbols
B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
UniProt Protein Name
B7-2
UniProt Synonym Protein Names
B7-2
Protein Family
T-lymphocyte activation antigen
UniProt Gene Name
Cd86
UniProt Entry Name
Q62810_RAT
NCBI Protein Information
T-lymphocyte activation antigen CD86; activation B7-2 antigen; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1

UniProt Comments for CD86

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: cell surface; intracellular membrane-bound organelle; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: negative regulation of T cell anergy; B cell activation; immune system process; toll-like receptor signaling pathway; positive regulation of T cell proliferation; positive regulation of activated T cell proliferation; defense response to virus
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Research Articles on CD86

1. novel function of CTLA4Ig in tumor immunity and suggest that CD86 on NK cells is an activating receptor and closely involved in the CTLA4Ig-mediated anti-tumor response.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CD86 elisa kit

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Organs/Tissues associated with CD86 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >279 publications with CD86 and Liver
 Eye Antibodies  >89 publications with CD86 and Eye
 Colon Antibodies  >71 publications with CD86 and Colon
 Placenta Antibodies  >43 publications with CD86 and Placenta
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