Methotrexate (MTX) ELISA Kit for Rat

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofMTXin the sample, This MTXELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusMTXconcentration. The concentration ofMTXin the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of MTX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS3808022 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Methotrexate (MTX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MTX. The ELISA analytical biochemical technique of the MBS3808022 kit is based on MTX antibody-MTX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MTX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MTX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).