Prolactin (PRL) ELISA Kit for Rat

Name: Rat PRL ELISA Kit; Prolactin; MyBioSource
Brand: MyBioSource
SKU: MBS2019961
Price: 455 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS2019961

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin (PRL). Standards or samples are then added to the appropriate microtiter plate wells… More details

$455 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Prolactin (PRL), ELISA Kit

Full Product Name

Rat Prolactin (PRL) ELISA Kit

Product Gene Name

PRL elisa kit

Species Reactivity

Rat

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Detection Range

0.312-20ng/mL

Sensitivity

0.127ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Prolactin (PRL).
No significant cross-reactivity or interference between Prolactin (PRL) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

25,876 Da

Chromosome Location

Chromosome: 6; NC_000006.12 (22287244..22302853, complement). Location: 6p22.3

OMIM

176760

3D Structure

ModBase 3D Structure for P01236

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Prolactin (PRL) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Prolactin (PRL) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for PRL elisa kit

Endocrinology; Reproductive science; Genetic science; Hormone metabolism

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of PRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRL purchase

MBS2019961 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prolactin (PRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRL. The ELISA analytical biochemical technique of the MBS2019961 kit is based on PRL antibody-PRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PRL. It may not necessarily be applicable to this product.

NCBI GI #

254675133

NCBI GeneID

5617

NCBI Accession #

NP_001157030.1

UniProt Primary Accession #

P01236

UniProt Secondary Accession #

Q15199; Q92996

UniProt Related Accession #

P01236

GenBank Nucleotide #

NM_001163558.2

NCBI Official Full Name

prolactin

NCBI Official Synonym Full Names

prolactin

NCBI Official Symbol

PRL

UniProt Protein Name

Prolactin

Protein Family

Prolactin

UniProt Gene Name

PRL

UniProt Synonym Gene Names

PRL

UniProt Entry Name

PRL_HUMAN

NCBI Protein Information

prolactin; decidual prolactin

NCBI Summary for PRL

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

UniProt Comments for PRL

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region

Molecular Function: protein binding; prolactin receptor binding; hormone activity

Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; regulation of multicellular organism growth; positive regulation of JAK-STAT cascade; female pregnancy

Research Articles on PRL

1. This study evaluated the expression of prolactin in the extrapituitary tissues of mice and its effect on infertility in mice with a prolactin knockout.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRL elisa kit

Reference Product	PubMed Publications
FSH elisa kit	>2307 publications with PRL and FSH
PRLR elisa kit	>324 publications with PRL and PRLR
JAK2 elisa kit	>179 publications with PRL and JAK2
POU1F1 elisa kit	>139 publications with PRL and POU1F1
STAT3 elisa kit	>56 publications with PRL and STAT3
GHR elisa kit	>52 publications with PRL and GHR
ESR1 elisa kit	>10 publications with PRL and ESR1
PTPN11 elisa kit	>6 publications with PRL and PTPN11

Pathways associated with PRL elisa kit

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Amyloids Pathway antibodies	Amyloids Pathway Diagram
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Disease Pathway antibodies	Disease Pathway Diagram
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Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram
Growth Hormone Receptor Signaling Pathway antibodies	Growth Hormone Receptor Signaling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with PRL elisa kit

Disease Name	Pubmed Publications
Pituitary Neoplasms Antibodies	>1987 publications with PRL and Pituitary Neoplasms
Hyperprolactinemia Antibodies	>1484 publications with PRL and Hyperprolactinemia
Adenoma Antibodies	>1460 publications with PRL and Adenoma
Prolactinoma Antibodies	>655 publications with PRL and Prolactinoma
Mental Disorders Antibodies	>587 publications with PRL and Mental Disorders
Amenorrhea Antibodies	>426 publications with PRL and Amenorrhea
Galactorrhea Antibodies	>314 publications with PRL and Galactorrhea
Growth Hormone-Secreting Pituitary Adenoma Antibodies	>258 publications with PRL and Growth Hormone-Secreting Pituitary Adenoma
Infertility, Female Antibodies	>228 publications with PRL and Infertility, Female
Hypogonadism Antibodies	>201 publications with PRL and Hypogonadism

Organs/Tissues associated with PRL elisa kit

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>488 publications with PRL and Testis
Uncharacterized Tissue Antibodies	>1 publications with PRL and Uncharacterized Tissue