SST ELISA Kit for Rat

Description: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of SST in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat SST. During the reaction, Rat SST in the sample or standard competes with a fixed amount of Rat SST on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat SST. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat SST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of SST elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2503580 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SST, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SST. The ELISA analytical biochemical technique of the MBS2503580 kit is based on SST antibody-SST antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SST antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SST. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).