Syntaxin-1a (STX1A) ELISA Kit for Rat

Name: Rat STX1A ELISA Kit; Syntaxin-1a; MyBioSource
Brand: MyBioSource
SKU: MBS2804377
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2804377

MBS2804377 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Syntaxin-1A (STX1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Syntaxin-1A (STX1A), ELISA Kit

Full Product Name

Rat Syntaxin-1A (STX1A) ELISA Kit

Product Gene Name

STX1A elisa kit

Product Synonym Names

HPC-1; STX1; p35-1; syntaxin 1A

Species Reactivity

Rat

Assay Type

Quantitive Sandwich

Samples

Serum, Plasma, Other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.078ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat STX1A. No significant cross-reactivity or interference between Rat STX1A and analogues was observed.

Preparation and Storage

Store at 2-8 degree C. Please refer to Instruction Manual.

Molecular Weight

33,067 Da

3D Structure

ModBase 3D Structure for P32851

Detection Wavelength

450nm

Assay Duration

1-4.5h

Sample Volume

1-200uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV

Test Principle

This assay employs a two-site sandwich ELISA to quantitate STX1A in samples. An antibody specific for STX1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STX1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STX1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STX1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of STX1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for STX1A. It may not necessarily be applicable to this product.

NCBI GI #

417842

NCBI GeneID

116470

NCBI Accession #

P32851.1

UniProt Primary Accession #

P32851

UniProt Related Accession #

P32851

NCBI Official Full Name

Syntaxin-1A

NCBI Official Synonym Full Names

syntaxin 1A

NCBI Official Symbol

Stx1a

UniProt Protein Name

Syntaxin-1A

UniProt Synonym Protein Names

Neuron-specific antigen HPC-1; Synaptotagmin-associated 35 kDa protein; P35A

Protein Family

Syntaxin

UniProt Gene Name

Stx1a

UniProt Synonym Gene Names

Sap; P35A

UniProt Entry Name

STX1A_RAT

NCBI Protein Information

syntaxin-1A

NCBI Summary for STX1A

plays a role in neurotransmitter release [RGD, Feb 2006]

UniProt Comments for STX1A

Plays a role in hormone and neurotransmitter exocytosis (PubMed:17301173, PubMed:18167541, PubMed:20484665, PubMed:22411134). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.

Research Articles on STX1A

1. Measuring rapid interaction dynamics during synaptic depolarization identified the small number of syntaxin1a and munc18-1 protein molecules required to support synaptic vesicle exocytosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with STX1A elisa kit

Reference Product	PubMed Publications
Snap25 elisa kit	>104 publications with STX1A and Snap25
Vamp2 elisa kit	>35 publications with STX1A and Vamp2
Stxbp1 elisa kit	>24 publications with STX1A and Stxbp1
Syt1 elisa kit	>16 publications with STX1A and Syt1
Vamp3 elisa kit	>10 publications with STX1A and Vamp3
Stxbp5 elisa kit	>6 publications with STX1A and Stxbp5
Vamp8 elisa kit	>6 publications with STX1A and Vamp8
Vamp1 elisa kit	>6 publications with STX1A and Vamp1
Stx8 elisa kit	>2 publications with STX1A and Stx8
Cplx1 elisa kit	>2 publications with STX1A and Cplx1

Pathways associated with STX1A elisa kit

Products by Pathway	Pathway Diagram
Acetylcholine Neurotransmitter Release Cycle Pathway antibodies	Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies	GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Glutamate Neurotransmitter Release Cycle Pathway antibodies	Glutamate Neurotransmitter Release Cycle Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
LGI-ADAM Interactions Pathway antibodies	LGI-ADAM Interactions Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Neurotransmitter Release Cycle Pathway antibodies	Neurotransmitter Release Cycle Pathway Diagram

Diseases associated with STX1A elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>34 publications with STX1A and Nervous System Diseases
Neurobehavioral Manifestations Antibodies	>16 publications with STX1A and Neurobehavioral Manifestations
Fibrosis Antibodies	>14 publications with STX1A and Fibrosis
Heart Diseases Antibodies	>13 publications with STX1A and Heart Diseases
Diabetes Mellitus Antibodies	>8 publications with STX1A and Diabetes Mellitus
Disease Models, Animal Antibodies	>7 publications with STX1A and Disease Models, Animal
Nerve Degeneration Antibodies	>3 publications with STX1A and Nerve Degeneration
Movement Disorders Antibodies	>3 publications with STX1A and Movement Disorders
Necrosis Antibodies	>3 publications with STX1A and Necrosis
Cognition Disorders Antibodies	>2 publications with STX1A and Cognition Disorders

Organs/Tissues associated with STX1A elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>156 publications with STX1A and Brain
Embryonic Tissue Antibodies	>28 publications with STX1A and Embryonic Tissue
Eye Antibodies	>14 publications with STX1A and Eye
Lung Antibodies	>9 publications with STX1A and Lung
Heart Antibodies	>6 publications with STX1A and Heart
Pituitary Gland Antibodies	>5 publications with STX1A and Pituitary Gland
Uncharacterized Tissue Antibodies	>3 publications with STX1A and Uncharacterized Tissue